In-Vitro Analysis of Extracts of Fresh Fruits and their Precaps Against Pathogenic Microbes
Keywords:
Antimicrobial activity, Manilkara zapota, Trigonella foenum-graecum, Vitis vinifera, Anthocephalus cadambaAbstract
Phytochemicals present in plants are of great significance to humans in various ways. They contribute life sustaining properties and have a potential to provide vigor against diseases viz. cancer and cardiovascular diseases. The objective of this analysis was to acquire data about the anti-microbial activity of fruits and vegetables against different pathogens viz. Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis. The extracts were prepared via solvent extraction method by dissolving different samples of fruits and vegetables in solvents viz. Acetone, Chloroform, 80% Methanol and 70% Ethanol for 48 hours. These solvents were kept under dark condition for two days so that the solvent retains its efficiency. Samples were then subjected to Anti-microbial susceptibility test and the analysis revealed that pre-cap of Vitis vinifera (Black grapes) gave the best result against Pseudomonas aeruginosa with a diameter of 23.50 mm. It was observed that the extract of Anthocephalus cadamba (Cadamba) gave better results when dissolved in polar solvents viz. 80% Methanol and 70% Ethanol than in Chloroform. Fresh extract of Manilkara zapota (chiku) gave results only when dissolved in Chloroform against all the three pathogens.
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