Murine Monoclonal Antibodies against the Antimicrobial Peptide Oreoch-2 from Tilapia (Oreochromis niloticus)

Authors

  • Maylin Pérez Bernal Center for Genetic Engineering and Biotechnology of Sancti Spiritus, Cuba
  • Onel Valdivia Center for Genetic Engineering and Biotechnology of Sancti Spiritus, Cuba
  • Reinaldo Blanco Center for Genetic Engineering and Biotechnology of Sancti Spiritus, Cuba
  • Joel Pérez Center for Genetic Engineering and Biotechnology of Sancti Spiritus, Cuba
  • Andy Domínguez Center for Genetic Engineering and Biotechnology of Sancti Spiritus, Cuba
  • Liliana Basabe Center for Genetic Engineering and Biotechnology of Havana, Cuba
  • Rebeca Martínez Center for Genetic Engineering and Biotechnology of Havana, Cuba
  • Yeosvany Cabrera Center for Genetic Engineering and Biotechnology of Sancti Spiritus, Cuba

Keywords:

ascitic fluid, cell fusion, ELISA, hybridoma

Abstract

Classic hybridoma technology has developed monoclonal antibodies (mAb) against a wide variety of antigens with several applications in biotechnology. In this work, murine mAb secreting hybridomas were generated for specific recognition of antimicrobial peptide Oreoch-2, isolated from tilapia and with demonstrated antimicrobial activity against bacteria and fungi. BALB/c mice were immunized with Oreoch-2 conjugated to bovine albumin. Cell fusion was performed between lymphocytes isolated from spleen of mouse with highest antibody serum titers and P3/x63.Ag8.653 myeloma cells. Three hybridomas with stable secretion of anti-P22 mAb were obtained, which did not show cross-reactivity with Oreoch-1 related peptide. The mAbs will be used in the study of the action mechanisms of antimicrobial peptide Oreoch-2 and to establish analytical tools for its detection and quantification in pharmaceutical formulations.

 

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Published

2021-02-28

How to Cite

[1]
M. P. Bernal, “Murine Monoclonal Antibodies against the Antimicrobial Peptide Oreoch-2 from Tilapia (Oreochromis niloticus)”, Int. J. Sci. Res. Biol. Sci., vol. 8, no. 1, pp. 32–35, Feb. 2021.

Issue

Vol. 8 No. 1 (2021): February Edition

Section

Research Article

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