Assessment of endophytic fungal isolates for its Antibiofilm activity on Pseudomonas aeruginosa
Keywords:
Endophytic fungi, Biofilm, Quorum sensing, Pseudomonas aeruginosaAbstract
The present study reports assessment of the antibiofilm activity of endophytic fungi on quorum sensing controlled biofilm formation in Pseudomonas aeruginosa MTCC 2453. Endophytic fungi isolated from agriculture field were evaluated for antibiofilm activity. After fermentation in sucrose broth, biofilm formation inhibitors from endophytic fungi were extracted using ethyl acetate. The active extract from each endophytic fungus was assayed for inhibition of biofilm formation using biosensor strain Pseudomonas aeruginosa. At highest tested concentration (200µg/ml) of both fungal extract and biocidal agent, the extract from Fusarium sp.1 demonstrated maximum inhibition in biofilm formation (49.65%) and minimum inhibition by Colletotrichum sp. extract (18.21%) and inhibition was significant at (P < 0.05). Similarly at highest tested concentration (200µg/ml) of fungal extract alone, the maximum percent decrease (18.90%) was observed in P. aeruginosa by Phoma sp. extract and minimum inhibition by Penicillium sp extract (4.00%) with no statistical difference. On the other hand extract from Aspergillus sp.2 was found to show no antibiofilm activity but exhibit stimulatory effect on biofilm formation extract (-4.78%). Combined effect of active fungal extract and biocidal agent resulted in significantly more percent biofilm inhibition as compared to fungal extract alone. The action of one or more active compounds extracted from endophytic fungi may be responsible for antibiofilm activity. Such compounds could be effective against emerging multidrug resistant pathogens.
References
C. Potera, “Forging a Link Between Biofilms and Disease,” Science. vol. 283: 1837–1839, 1999.
M. S. P. B. D and S. Yadav, “Biofilms : Microbes and Disease,” Brazilian Journal of Infectious Diseases vol, 12, pp. 526–530, 2008
J. W. Costerton, P. S. Stewart, and E. P. Greenberg, “Bacterial Biofilms : A Common Cause of Persistent Infections,” Science, vol. 284, no. May, pp. 1318–1322, 1999.
T. I. M. Tolker-nielsen, U. C. Brinch, P. C. Ragas, J. B. O. Andersen, C. S. Jacobsen, and S. Molin, “Development and Dynamics of Pseudomonas sp, Biofilms,” Journal of Bacteriology, vol. 182, no. 22, pp. 6482–6489, 2000.
K. Sauer et al., “Pseudomonas aeruginosa Displays Multiple Phenotypes during Development as a Biofilm Pseudomonas aeruginosa Displays Multiple Phenotypes during Development as a Biofilm,”, Journal of Bacteriology, 184:1140–54. 2002.
M. G. Kociolek, “Quorum-Sensing Inhibitors and Biofilms,” Journal of Medicinal Chemistry, vol. 8, no.4, pp. 315–326, 2009.
W. Yao, D. A. I. Yue, Z. Yong, H. U. Yangbo, Y. Baoyu, and C. Shiyun, “Effects of quorum sensing autoinducer degradation gene on virulence and biofilm formation of Pseudomonas aeruginosa,” Science in China Series C Life Sciences, vol. 50, no. 30570020, 2007.
C. Van Delden and B. H. Iglewski, “Cell-to-Cell Signaling and Pseudomonas aeruginosa Infections,” Emerging Infectious Dieasess. vol. 4, no. 4, pp. 551–560, 1998.
L. B. Rice, “Federal Funding for the Study of Antimicrobial Resistance in Nosocomial Pathogens : No ESKAPE,” The Journal of Infectious Diseases, vol. 197, pp. 1079–1081, 2008.
Macé, C, D. Seyer, C. Chemani, P. Cosette, P. Di-Martino et al., “Identification of Biofilm-Associated Cluster ( bac ) in Pseudomonas aeruginosa Involved in Biofilm Formation and Virulence,” PLOS.vol. 3, no. 12, pp. 1–10, 2008.
U. S. E. Arivudainambi, T. D. Anand, V. Shanmugaiah, C. Karunakaran, and A. Rajendran, “Novel Bioactive metabolites producing endophytic fungus Colletotrichum gloeosporioides against multidrug-resistant Staphylococcus aureus,” FEMS Immunology & Medical Microbiology, vol. 61, pp. 340–345, 2011.
R. X. Tan and W. X. Zou, “Endophytes : a rich source of functional metabolites,” Natural Products Reports, no. 18, pp. 448–459, 2001.
W. X. Zou et al., “Metabolites of Colletotrichum gloeosporioides , an Endophytic Fungus in Artemisia mongolica,” Journal of Natural Products, vol. 63, no. 11. 1529–1530, 2000.
J. Buatong and S. Phongpaichit, “Antimicrobial activity of crude extracts from mangrove fungal endophytes,” World Journal Microbiology and Biotechnology no. 27 pp. 3005–3008, 2011.
N. Radic and B. Strukelj, “Phytomedicine Endophytic fungi -The treasure chest of antibacterial substances,” Phytomedicine vol. 19, pp. 1270–1284, 2012.
A. J. Martín-Rodríguez et. al., “Aquatic Fungi : First Report from Marine Endophytes,” Marine Drugs, no.12, pp. 5503–5526, 2014.
P. S. Rajesh and V. R. Rai, “Biocatalysis and Agricultural Biotechnology Hydrolytic enzymes and quorum sensing inhibitors from endophytic fungi of Ventilago madraspatana Gaertn,” Biocatalysis Agricultural Biotechnology., vol. 2, no. 2, pp. 120–124, 2013.
M. Ueda, T. Kubo, and K. Miyatake, “Purification and characterization of fibrinolytic alkaline protease from Fusarium sp . BLB,” Applied Microbiology and Biotechnology, vol. 74 pp. 331–338, 2007.
J. C. Taganna, J. P. Quanico, R. Marie, G. Perono, E. C. Amor, and W. L. Rivera, “Tannin-rich fraction from Terminalia catappa inhibits quorum sensing ( QS ) in Chromobacterium violaceum and the QS-controlled biofilm maturation and LasA staphylolytic activity in Pseudomonas aeruginosa,” Journal of Ethnopharmacology, vol. 134, pp. 865–871, 2011.
S. S. Meenambiga and K. Rajagopal, “Antibiofilm activity and molecular docking studies of bioactive secondary metabolites from endophytic fungus Aspergillus nidulans on oral Candida albicans,” Journal of Applied Pharmaceutical Science, vol. 8, no. 3, pp. 37–45, 2018.
C. Nithya, F. Lewisoscar, S. Kanaga, R. Kavitha, and D. Bakkiyaraj, “Biofilm inhibitory potential of Chlamydomonas sp. extract against Pseudomonas aeruginosa,” Journal of Algal Biomass Utilization, vol. 5, no. 4, pp. 74–81, 2014.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors contributing to this journal agree to publish their articles under the Creative Commons Attribution 4.0 International License, allowing third parties to share their work (copy, distribute, transmit) and to adapt it, under the condition that the authors are given credit and that in the event of reuse or distribution, the terms of this license are made clear.
Download Article Template
