Comparative Evaluation of Qualitative and Quantitative Phytochemical Analysis of South Indian Bioactive Medicinal Plant Tribulus terrestris
Keywords:
Tribulus terrestris, Phytochemical screening, Leaf Extract, Secondary MetabolitesAbstract
In India researchers are focused on plant medicine to avoid the use of synthetic antibiotics. About 80% of rural population depends on herbal medicine for their primary health care. Due to the inadequacy of information about their phytochemical and therapeutic properties, some of these sources are still remained unrevealed. In this present study Tribulus terrestris (TT) was collected from local area in and around Gobichettipalayam, Erode and Tiruppur District, India. The qualitative phytochemical analysis of Tribulus terrestris (TT) was performed in seven solvents of Chloroform, Petroleum ether, Acetone, Methanol, Ethanol, Ethyl acetate and Aqueous extracts. The phytochemical analysis of crude extracts of Tribulus terrestris showed the presence of carbohydrates, amino acids, proteins, glycosides, tannins, terpenoids, phenols, alkaloids and saponins. Each crude extract of the plant Tribulus terrestris was comparatively analyzed for presence of phytoconstituents and ethyl acetate has good extractability. The qualitative estimation of Tannins, Alkaloids, and Flavonoids in the Ethyl acetate extract of Tribulus terrestris was determined by the standard methods. The total Tannin, Alkaloid and Flavonoid content of the Ethyl acetate extracts were 2.76µg/ml,0.56mg/ml and 0.45mg/ml. The thin layer chromatography was performed to find most active constituent in all fractions.
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