Isolation and Characterization of Bacteria for Bio-surfactant production from oil-contaminated and Ffarmland Soil in Dutsin-Ma L.G.A
Keywords:
Biosurfactants, Bacteria, Soil, Screening, Dutsin-MaAbstract
Surface-active substances that are secreted by microorganisms are called biosurfactants. Although there are a number of known microorganisms that create biosurfactants, more research is needed to determine which ones could produce the chemical more quickly and profitably for commercial usage. These kinds of bacteria were identified in the Dutsin-Ma metropolitan investigation as being involved in the production of biosurfactant. Six soil samples in all were gathered from various locations within the Dutsin-Ma city. Six soil samples in all were gathered from various locations within the Dutsin Ma city. The tests included isolating the bacteria, identifying the group to which the organisms belong using gram staining, testing the isolated bacteria biochemically, and performing additional tests to identify bacteria that create biosurfactants. Prior to microscopic examination, the bacteria used in this investigation were first acquired macroscopically. It was noted that several colony characteristics included colour, texture, border, and height. It was noted that B. subtilis had an entire border, a smooth texture, and a yellowish coloration. The isolates` biochemical examination reveals Bacillus subtilis, Pseudomonas sp., and pseudomonas aeruginosa. The fact that the bacteria are there indicates that the soil is a natural habitat for them. based on the biosurfactant activity screening using the oil spread, drop collapse, and haemolysis tests. Every isolate underwent a hemolysis test, and every one of them proved positive (Pseudomonas spp., however, had a negative result). The haemolysis result demonstrates beta hemolysis activity, a sign of the strain`s capacity to generate biosurfactants. With the exception of pseudomonas sp., all isolates showed positive for the drop collapse and oil spread tests. Further research is required to determine the precise features of the type and structure of the biosurfactant that these bacteria eat, based on the results of this study.
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