Determination of Anti-Inflammatory Activity of Coumarin Compounds of Coldenia Procumbens Linn
Keywords:
C.procumbens, coumarin, BSA, Egg albuminAbstract
In the present study, invitro anti-inflammatory activity was tested using coumarin compound of C.procumbens. Coumarins are a group of polyphenolic compounds isolated by TLC method from C.procumbens plant extract. In anti-inflammation study, the methanolic extract showed high percentage of inhibition observed in egg albumin denaturation studies was 78.8% of 0.8µg/ml concentration of coumarin compounds where as compared to standard drug Diclofenac sodium 74.8%. While, bovine serum albumin denaturation assay was 67.8 % of 0.8 µg/ml concentration when compared to other concentration of coumarin compounds. The results were discussed.
References
Amri, O., Zekhnini, A., Bouhaimi, A., Tahrouch, S. and Hatimi, A., (2018). Anti-inflammatory activity of methanolic extract from Pistacia atlantica Desf. leaves. Pharmacog J.; 10(1):71-6.
Chatterjee P, Chanda S, Dev P., and Bhattacharya S. (2012). Evaluation of anti-inflammatory effects of green tea and black tea: a comparative in vitro study. Journal of Advanced Pharmaceutical Technology and Research.; 3(2):136-138.
Chopra, R.N., Nayar, S.L. and Chopra, I.C., (1956). Glossary of Indian Medicinal Plants. CSIR, New Delhi.
Cox, P.A. and Balick, M.J., (1994). The ethnobotanical approach to drug discovery. Scientific American.; 270(6):60-65.
Das, S.N. and Chatterjee, S., (1995). Long term toxicity study of ART‐400. Indian Indg Med; 16 (2): 117‐123
Dinarello C., (2010). Anti-inflammatory Agents: Present and Future. Cell; 140: 935–950.
Heendeniya, SN., Ratnasooriya WD. and Pathirana, RN., (2018). In vitro investigation of anti-inflammatory activity and evaluation of phytochemical profile of Syzygium caryophyllatum. J. of Pharmaco. and Phytochem.; 7(1): 1759-1763.
Kennedy and Wightman. (2011). Herbal extracts and phytochemicals: plant secondary metabolites and the enhancement of human brain Function1. Advance in. Nutrition.; 2:32-50.
Kiritikar, K.R. and Basu, B.D., (1980). Indian Medicinal Plants, V. 1-4. Bishen Singh Mahendra Pal Singh, Dehra Dun.
Leelaprakash G. and Mohan Dass S., (2011). “Invitro Antiinflammatory activity of Methanol extract of Enicostemma Axillare. Int. J. Drug Dev. and Res., 3(3): 189-196.
Medzhitov R., (2010). Inflammation: New Adventures of an Old Flame. Cell; 140: 771–776.
Mizushima Y. and Kobayashi M., (1968). Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. Pharmacy and Pharmacology.; 20:169-73.
Mizushima Y., (1966). Screening test for anti-rheumatic drugs. Lancet.; 2:443-448.
Muratovic, S. Osmanovic, A., Veljovic, E., Dzudzevic-Cancar, H., Duric, K., Niksic, H. and Zavrsnik, (2013). Evaluation of purity of some coumarin derivatives by melting point measurements, TLC and scanning densitometry. Bulletin of the Chemists and Technologists of Bosnia and Herzegovina.: 41: 15-19
Nyiredy, S. (2001). Planar Chromatography a Retrospective View for the Third Millenium. 36-42. Springer Scientific Publisher, Budapest.
Rahman, H., Eswaraiah, M.C. and Dutta, A.M., (2015). In-vitro antiinflammatory and anti-arthritic activity of Oriza sativa Var. Joha Rice (An Aromatic Indigenous Rice of Assam). American Eurasian Journal of Agricultural and Environmental Sciences.; 15(1):115-121.
Saso, L, Valentini, G, Casini, ML, Grippa, E, Gatto, MT. and Silvestrini, B. (2001). Inhibition of heat-induced denaturation of nonsteroidal antiinflammatry drugs (NSAIDs): pharmacological implications. Archives of Pharma. Research.; 24(2):150-158.
Umapathy, E., Ndebia, E.J., Meeme, A., Adam, B., Menziura, P., Nkeh-Chungag, B.N. and Iputo, J.E., (2010). An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. J.of Medicinal Plant Res.; 4 (5) 789-795.
Vane, J.R. and Botting, R.M., (1995). New insights into the mode of action of anti-inflammatory drugs. Inflammation Res.; 44 (1) 1-10.
Williams L.A., O'Connar, A., Latore, L., Dennis, O., Ringer, S., Whittaker, J.A., Conrad, J., Vogler, B., Rosner, H. and Kraus, W., (2008). The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process. West Indian Med J. ;57(4):327-31.
Zhao HL, Harding SV, Marinangeli CP, Kim YS. and Jones PJ., (2008). Hypocholesterolemic and anti-obesity effects of saponins from Platycodon grandiflorum in hamsters fed atherogenic diets. Journal of Food Science.; 73(8):195-200.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors contributing to this journal agree to publish their articles under the Creative Commons Attribution 4.0 International License, allowing third parties to share their work (copy, distribute, transmit) and to adapt it, under the condition that the authors are given credit and that in the event of reuse or distribution, the terms of this license are made clear.
Download Article Template
