Antifungal activity of Streptomyces longisporoflavus isolated from Lonar Lake against Alternaria solani
Keywords:
Antifungal activity, Alternaria solani, Lonar Lake, Streptomycetes, disease managementAbstract
Actinomycetes were isolated from water samples of Lonar Lake, Buldhana district of Maharashtra, India and screened for their antifungal activity by agar well diffusion method against phyto-pathogenic fungus Alternaria solani. The strain LL-5 showing highest antifungal activity was identified as Streptomyces longisporoflavus on the basis of microscopic and biochemical characteristics. The antifungal metabolite production using S. longisporoflavus was optimized based on optimal incubation period, temperature, initial pH of medium and supplementation of carbon and nitrogen sources. The study revealed that incubation period of 8 days, 30ᴼC, pH 10.0, starch (1%) as carbon source and peptone (0.5%) as nitrogen source are favorable parameters for maximum production of antifungal metabolite by S. longisporoflavus. These results clearly elucidates that S. longisporoflavus isolated from Lonar Lake water sample could be a good source of antifungal metabolite that can be used for management of diseases caused by Alternaria solani.
References
K.K. Pandey, P.K. Pandey, G. Kalloo, M.K. Banerjee, “Resistance to early blight of tomato with respect to various parameters of disease epidemics”, J. General Pl. Pathol., Vol.69, pp.364-371, 2003.
M. Olanya, C. Honeycutt, R.P. Larkin, T.S. Griffin, Z. He, J.M. Halloran, “The effect of cropping systems and irrigation management on development of potato early blight”. J. Gen. Plant Pathol, Vol.75, pp.267-275, 2009.
F. Samen, S.J. Goussous, A. Shudifat., I. Makhadmeh, “Reduced sensitivity of tomato early blight pathogen (Alternaria solani) isolates to protectant fungicides and implications on disease control”. Archives of phytopathology and plant protection., Vol.49, pp.120-136, 2016.
J.G. Holt, N.R. Krieg, P.H.A. Sneath, J.T. Staley, S.T. Williams, “Bergey’s Manual of Determinative Bacteriology”, 9th edn. Baltimore, MD: The Williams and Wilkins Company, 1994.
B.M. Gibbs, D.A. Shapton DA (eds) (1968). Identification methods for microbiologists, part B, London: Academic press. Pg 111, 1968.
B. Becker, M.P. Lechevalier, R.E. Gordon, H.A. Lechevalier, “Paper Chromatography of whole-cell hydrolysates”, Appl. Microbiol., Vol.12, pp.421-423, 1964.
M.P. Lechevalier, “Identification of aerobic actinomycetes of clinical importance”. J. Lab. Clin. Med. Vol.71, pp.934-944, 1968.
N. Gupta N, S. Mishra, U.C. Basak, “Diversity of Streptomyces in mangrove ecosystem of Bhitarkanika”, Iranian J Microbiol, Vol.1, pp.37-42, 2009.
H.E. Muhsin, A.A Bazzaz, “Identification of Streptomyces spp and assessment of their inhibiting metabolic potency against some pathogenic microorganisms”. Merit Res J Med. Med Sci, Vol.3, pp.511-516, 2015.
P.N. Arusha, D.D Karad, S.G. Gupta, A.S. Kharat, “Consortium inoculum of five thermotolerant phosphate solubilizing actinomycetes for multipurpose biofertilizer preparation”, Iranian J Microbiol, Vol.9, pp.295-304, 2017.
R. Vijayakumar, K. Panneerselvam , C. Muthukumar, N. Thajuddin, A. Panneerselvam et al, “Optimization of antimicrobial production by a marine actinomycete Streptomyces afghaniensis VPTS3-1 isolated from Palk Strait, East Coast of India”, Indian J Microbiol., Vol.52, pp.230-239, 2012.
H.J. Bhosale, T.A. Kadam, R.S. Mirajgave, S.K. Holkar, “Optimization and characterization of antifungal metabolite from a soil actinomycete Streptomyces indiaensis SRT-1”. Ind J Biotechnol, 17, 2018 (In press).
K. Kathiresan, R. Balagurunathan, S.M. Masilamani, “Fungicidal activity of marine actinomycetes against phytopathogenic fungi”, Ind J Biotechnol., Vol.4, pp.271-276, 2005.
S. Omura, A. Nakagawa, H. Yamada, T. Hata, A. Furusaki, T.C. Wtanabe, “Structure and biochemical properties of kanamycin A, B, C and D”. Chemical and Pharmaceutical Bulletin, Vol.21, pp. 931-934, 1973.
M.K. Gupta, A. Pande, “Effect of physiological and physical factors on antifungal activities of Streptomyces spp”. J Ind Bot Soc, Vol.72, pp.157–159, 1992.
H.J. Bhosale, T.A. Kadam, S.G. Fulwad, M.A. Karale, O.S. Kanse, “Optimization of antifungal compound production by moderately halophilic Str. werraensis HB-11”, Int J Pharm Sci Res, Vol.6, pp.1090-99, 2015.
I.M. Ababutain, Z.K. Abdul Aziz, N.A. Al-Meshhen, “Optimization of environmental and nutritional conditions to improve growth and antibiotic production by Streptomyces sp. isolated from Saudi Arabia soil”. Int Res J Microbiol, Vol.4, pp. 179-187, 2013.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors contributing to this journal agree to publish their articles under the Creative Commons Attribution 4.0 International License, allowing third parties to share their work (copy, distribute, transmit) and to adapt it, under the condition that the authors are given credit and that in the event of reuse or distribution, the terms of this license are made clear.
Download Article Template
