A Comparative Study between Plant and Callus Extracts: Analysis of Phytochemical, Antioxidant, Antibacterial and Anticancer Properties of Ormocarpum Sennoides (Willd.) DC
Keywords:
In vivo plant, callus, phytochemicals, antioxidants, anticancerAbstract
A comparative study was conducted between the in vivo grown plant and in vitro callus of Ormocarpum sennoides (Willd.) DC using aqueous, methanol and ethyl acetate as solvents. MS medium supplemented with BAP 2.0 mg/L + IAA 3.0 mg/L has shown the highest callus induction percentage of 86.7% with the maximum callus weight of 1.03g. Ormocarpum sennoides contains a maximum of 7% total ash. The phytochemical analysis revealed that the aqueous, methanol and ethyl acetate extracts of both plant and callus showed the presence of many phytochemical compounds. FTIR analysis of plant and callus aqueous extract had a maximum characteristic band at 3403.18 cm -1 indicating the presence of O-H stretching and 3406.51 cm-1 indicating the presence of N-H stretching respectively. The GC-MS analysis of methanol extract of the plant indicates the presence of Alpha.-Methyl Mannofuranoside and Silane,[[(3.Beta.)-Gorgost-5-en-3-yl]oxy]Trimethyl-. Callus extract indicates the presence of D-Glucopyranoside, Methyl 3-O-Methyl, Ala-.Beta.-Ala, Trimethylsilyl ester and 4-Pentadecyne,15-chloro-. Plant extract exhibited maximum total phenol content and DPPH radical scavenging activity than callus extracts. Methanol and ethyl acetate plant showed a maximum zone of inhibition of 6 mm against Bacillus subtilis. While the ethyl acetate extract of both plant and callus showed a maximum zone of inhibition of 7mm against Escherichia coli. Aqueous extract of plant and callus showed a maximum zone of inhibition of 10mm and 9mm against Staphylococcus aureus respectively. Anticancer studies revealed that in vivo plant has the inhibitory percentage of 24.9% whereas callus exhibits only 9.3% as an inhibitory percentage against HeLa cells.
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