In Vitro Propagation of Endangered Medicinal Plant Ormocarpum Sennoides (Willd.) DC From Internodal Segments

Authors

  • G. Nandhini Department of Biotechnology, Stella Maris College (Autonomous), Chennai - 600 086, India
  • J. Anbumalarmathi Department of Biotechnology, Stella Maris College (Autonomous), Chennai - 600 086, India
  • K. Mercy Madhumitha Department of Biotechnology, Stella Maris College (Autonomous), Chennai - 600 086, India

Keywords:

Ormocarpum sennoides, endangered medicinal plant, hardening

Abstract

An efficient regeneration protocol was established for in vitro propagation through direct and indirect regeneration of Ormocarpum sennoides from internodal explants. Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyladenine amino purine (BAP), 2, 4 Dichlorophenoxy acetic acid (2,4-D), Indole-3-acetic acid (IAA) and Napthalene acetic acid (NAA) either alone or in combinations. Indirect regeneration, MS medium supplemented with D1: BAP 2.5 mg/L has shown the highest regeneration percentage of 88.9% with shoot length of 8.36 cm and root length of 1.52 cm. In indirect regeneration I4: BAP 2.0 mg/L + IAA 3.0 mg/L has shown the highest callus induction percentage of 86.7% and maximum callus weight of 1.03g. The highest frequency of shoot regeneration (68.3%) with maximum shoot length (3.53 cm) was observed in MS medium supplemented with IS5: BAP 3.5 mg/L. The highest frequency of root regeneration (56.1%) with maximum root length (1.53 cm) was observed in MS medium supplemented with IR5: IAA 2.5 mg/L + NAA 1.0 mg/L. The in vitro derived rooted plantlets were successfully hardened and were acclimatized in the greenhouse.

References

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Published

2020-02-28

How to Cite

[1]
G. Nandhini, J. Anbumalarmathi, and K. M. Madhumitha, “In Vitro Propagation of Endangered Medicinal Plant Ormocarpum Sennoides (Willd.) DC From Internodal Segments”, Int. J. Sci. Res. Biol. Sci., vol. 7, no. 1, pp. 84–89, Feb. 2020.

Issue

Vol. 7 No. 1 (2020): February Edition

Section

Research Article

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