Evaluation of Prodigiosin pigment for antimicrobial and insecticidal activities on selected bacterial pathogens & household pests.
Keywords:
Serratia, anti microbial, antifungal, insecticidalAbstract
Bacterial pigments are gaining commercial importance in various industries like food, textile, cosmetics, pharmaceutical and other industries. Bacterial pigments are advantageous over others as they are cost effective and eco friendly. Prodigiosin has been reported to have many applications such as antibacterial, antifungal, tumor suppressor, antimalarial, antiprotozoal, immunosuppressive etc. due to its natural red pigmentation. Applications of prodigiosin prompts one to develop a cost-effective bioprocess for its production. Multi-facilitated potency of prodigiosin pigment produced by Serratia could be explored not only for antibacterial and antifungal but also as effective biocontrol agent in agriculture. Present study therefore aimed to obtain a potential isolate of Serratia for prodigiosin production. Evaluation of the pigment was carried out with four bacterial strains, two fungal plant pathogens and four insect pests. Peanut broth when inoculated with the isolate could yield maximum pigment production at 300C for 72hours. The extracted pigment purity was confirmed by the spectrophotometric method and also by TLC. Prodigiosin was observed to be effective against E.coli and Staphylococcus with an MIC value of 400 µg.However it had no impact on Pseudomonas and Bacillus cultures. When compared with the standard Nistatin, MIC of the pigment was observed as 80 µg. for Alternaria and for Fusarium MIC was observed at 160 µg. Insecticidal activity 100 % mortality was found against Cockroaches and tropical ants while 85 -71% was observed with termites and pyramid ants.
References
Narsing Rao MP, Xiao M, Li W-J. Fungal and bacterial pigments: secondary metabolites with wide applications. Front Microbiol. Vol 8, pp 1113. 10.3389,2017
Heer K, Sharma S. Microbial pigments as a natural colour: a review. Int J Pharm Sci Res. 810.13040/IJPSR.0975-8232.8(5).1913-22, 2017
Shirata A, Tsukamoto P, Yasui H, Hata T, Hayasaka S, Kojima A, and Kato H (2000) Isolation of bacteria producing bluish purple pigment and use of dyeingJARQ.Vol 34, pp 131-140
Lewis S.M., Corpe W. A. Prodigiosin-Producing Bacteria from Marine Sources. Appl. Microbiol.,;Vol 12,No 1,pp 13–17,1964.
D’Aoust J.Y., Gerber N.N. Isolation and purification of prodigiosin from Vibrio psychroerythrus. J. Bacteriol., Vol118,No 2,pp756–757,1974.
Khanafari A, Assadi M.M., Fakhr F.A. Review of Prodigiosin, Pigmentation in Serratia marcescens. J. Biol. Sci., Vol6 No 1, pp 1–13,2006.
Giri V. Anuradha, NandhiniAnandkumar, Geetha muthukumaran and Gautham pennathur() A novel medium for enhancing cell growth and production of prodigiosin from Serratia marcescens isolated from soil, BMC Microbiol.Vol 4, pp11-18,2004.
Harris, A.K.P.; N.R.; Slater, H.;Cox, A.; Abbasi, S.;Foulds, I.;Simonsen, H.T.; Leeper, F.J.; Salmond, G.P.C. The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species – dependent genome context variation. Microbiol, Vol150,pp3547-3560,2004.
Boger, D.L. and M.Patel. Total synthesis of prodigiosin, prodigiosene and desmethoxyprodigiosin: Diels-Alder reactions of hetero cyclic azidenes and development of an effective palladium (II)-promoted 2`2`-bipyrrole coupling procedure. J. Org. Chem., Vol 53, pp1405-1415, 1988.
Kobayashi N., Ichikawa Y. Separation of the prodigios in localizing crude vesicles which retain the activity of protease and nuclease in Serratia marcescens. Microbiol. Immunol.;Vol35,No8, pp 607–14,1991.
Patil N. G., Kadam M. S., Patil V. R. and Chincholkar S. B. Insecticidal properties of water diffusible prodigiosin produced by Serratia nematodiphila 213C. Current Trends in Biotechnology and Pharmacy Vol. 7 pp 773-781 July 2013,
Martha Ingrid Gutiérrez-Román, Francisco Holguín-Meléndez, Michael F. Dunn, Karina Guillén-Navarro, Graciela Huerta-Palacios, Antifungal activity of Serratia marcescensCFFSUR-B2 purified chitinolytic enzymes and prodigiosin against Mycosphaerellafijiensis, causal agent of black Sigatoka in banana (Musaspp.), Vol 60, No 4, pp 565–572, 2015.
Darah Ibrahim1, TehFaridah Nazari1, Jain Kassim2, Sheh-Hong Lim Prodigiosin - An antibacterial red pigment produced by Serratia marcescens IBRL USM 84 associated with a marine sponge Xestospongia testudinaria , Journal of Applied Pharmaceutical Science Vol. 4 No 10, pp. 001-006, 2014
Phatake Y.B, Dharmadikari S.M MEvaluation of cytotoxic and anticancer activity of prodigiosin produced by Serratia Spp., Am. J. PharmTechRes.,Vol6, No3, 2016.
Estelle Marchal, Deborah A.Smithen, Md.Imam Uddin, Andrew W.Robertson, David L.Jakeman, Vanessa Mollard, Christopher D.Goodman Kristopher S.MacDougall, Sherri A.McFarland, Geoffrey McFadden, and Alison Thompson Synthesis and antimalarial activity of prodigiosenes Organic and biomolecular chemistry.Vol24,2014.
Gerber N.N., Lechevalier M.P. Prodiginine (prodigiosin-like) pigments from Streptomyces and other aerobic Actinomycetes. Can. J. Microbiol., Vol22,No5, pp 658–67,1976.
Gerber NN. ‘Prodigiosin like pigments’, CRC Critical Review of Microbiology, Vol3, pp 469-485,1975.
J. U. Vora, N. K. Jain and H. A. Modi. Extraction, Characterization and Application studies of red pigment of halophile Serratia marcescens KH1R KM035849 isolated from Kharaghoda soil.International Journal of Pure and Applied Bioscience. Vol 2,No 6, pp 160-168, 2014
Broekaert, W.F., F.R.G. Terras, B.P.A. Cammue, and J. Vanderleyden.An automated quantitative assay for fungal growth inhibition. FEMS Microbiology Letters Vol 69, pp 55-60,1990.
Eloff, J.N. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta MedicaVol 64, pp 711-713, 1998.
M. Parmasivam and C.Selvi Laboratory bioassay methods to assess the insecticide toxicity against insect pests – A review. Journal of entomology and zoology studies. Vol 5, No 3, pp 1441-1445,2017
Mangesh V. Suryavanshi, Samadhan R. Waghmode, Nidhi Bharti, Prafulla B. Choudhari, Tejashri B. Hingamire, Yogesh S. ShoucheIsolation and virtual screening of antimicrobial prodigiosin pigment from oxalotrophic Serratia marcescens OX_R strain Journal of Applied Pharmaceutical Science Vol. 6 No 11, pp. 052-058, 2016.
Griffin, G.F., and F.S. Chu. Toxicity of the Alternaria metabolites alternariol, alternariol methyl ether, altenuene, and tenuazonic acid in the chicken embryo assay. Applied and Environmental Microbiology Vol. 46, pp 1420-1422, 1983.
Goswami RS, Kistler HC. Heading for disaster: Fusarium graminearum on cereal crops. Molecular Plant Pathology. Vol 5, No 6, pp 515–25,2004.
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