Green synthesis of iron, copper and silver nanoparticles and their antibacterial activity on animal pathogens – A Comparative study
Keywords:
green synthesis, Azadirachta indica, nanoparticles, animal pathogensAbstract
Prevalence of antibiotic resistance in the animal pathogens is posing a threat to the treatment of various veterinary diseases like anthrax, brucellosis, gastritis caused by Bacillus anthracis, Brucella and Staphylococcus aureus. Antibiotic resistant bacteria may spread from animals to humans through various veterinary products. Challenges in the area of antibiotic resistance are met not only by combined drug therapy and the use of novel antimicrobial agents, but also the use of nanomedicine is offering an alternative to treat drug resistant pathogens. Nanoparticle research is currently an area of intense scientific research due to their unique properties like smaller size, penetrating ability into the cell and diversified applications in fields like biomedicine, agriculture, information technology. Since nanoparticles serve as potent antimicrobial agents, the present study mainly focuses on evaluating the efficacy of Iron, Copper and Silver nanoparticles as antimicrobial agents on animal pathogens. Green synthesis of nanoparticles from plant sources is the most emerging method as compared to the chemical and physical methods as it is less time consuming, cost effective and eco- friendly. Neem (Azadirachta indica) leaf extract was used to synthesis the Iron, Copper and Silver nanoparticles and were further characterized using techniques like UV-Vis Spectroscopy and SEM (Scanning Electron Microscopy). The synthesized nanoparticles were tested for antibacterial activity using well-diffusion assay against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) isolates obtained from P. V. Narsimha Rao State Veterinary University. Antibacterial study reveals that Silver nanoparticles synthesized using Neem leaf extract show significant antibacterial effect when compared to Iron and Copper nanoparticles.
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