Antibacterial activity of Hibiscus rosa sinensis and Calendula offcinalis flowers extract against various Pathogen

Abstract

From the long period of time, plants have been a valuable resource of natural products for human health . Some of plants leaves showing antimicrobial activity . The Primary benefit of using plant derived medicines is that they are readily affordable and accessible and showing fewer side effect . In the present study, I have evaluated that antibacterial activity of Hibiscus rosa sinensis flowers and Calendula offcinalis flowers extract against some clinical isolates of bacteria. The crude preparation of Hibiscus rosa- sinensis flowers were tested against Gram Positive- Staphylococcus aureus and Gram negative- Salmonella typhimurium, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa. Simultaneously Standard antibiotic solution of ampicillin is used. Calendula offcinalis flowers crude extract antimicrobial activity was evaluated against Gram Positive- Staphylococcus aureus, Bacillus subtilis. Gram negative - Pseudomonas aeruginosa , E.coli , Salmonella thyphi . Gram negative bacterium - Pseudomonas aeruginosa showed maximum zone of inhibition against Hibiscus rosa- sinensis flower extract and E.coli against Calendula offcinalis among these organisms. Simultaneously standard antibiotic solution of ampicillin was used. This experiment was done by agar gel diffusion method. The flower materials can be taken as an alternative source of antibacterial agent against the human pathogens. There may be possible use of these flowers extract for making drugs. Further pharmacological and clinical studies are required. These extract may be tested against multiple drug resistance microbes.