Conventional vegetative propagation and in vitro seed germination of endemic plant Colubrina travancorica Bedd
Keywords:
Colubrina travancorica Bedd., endemic plant, hardwood cuttings, seed germination, softwood cutting, rootingAbstract
Colubrina travancorica Bedd. is a medicinally important endemic plant species of Southern Western Ghats. Scientific studies are lacking due to the non availability of this Rare Endangered and Threatened (RET) species. For the conservation and establishment of C. travancorica, propagation methods have to be standardized. In the present study, propagation of the plant is achieved through hard wood cuttings, softwood cuttings and in vitro seed germination with different plant growth regulators. The type of auxins, Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA) or Naphthalene acetic acid (NAA) at varying concentrations affect the rooting response of hardwood and softwood cuttings. Significantly (p<0.001) high rooting was observed in hardwood cuttings treated with 2.5 mM IAA (53.3%). The number of roots (25.16) was significantly (p<0.001) high in cuttings treated with 0.5 mM NAA. Seasonal variation in the rooting response was observed in both hardwood and softwood cuttings. In soft wood cuttings maximum rooting (26.6%) was observed in 20 mM IBA treatment. Control set completely failed to root in the soft wood cuttings. Highest in vitro seed germination was achieved with 6.0µM Gibberellic acid. Among the various propagation methods hard wood cuttings are found to be the most suitable material for the propagation of C. travancorica and it can be effectively used for the large scale multiplication of this plant.
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