Production of cellulases through Solid State Fermentation (SSF) using agricultural waste biomass as solid substrates by Aspergillus niger
Keywords:
Aspergillus niger, Agricultural waste biomass, Cellulases, Solid State Fermentation (SSF)Abstract
A study was conducted to appraise the potential of using agricultural lignocellulosic wastes for cellulase production by Aspergillus niger through Solid State Fermentation (SSF) technique. In the present study, agricultural waste biomass like Banana Peels (BP), Cotton Stalks and Leaves (CSL), Green Pea Shell (GPS), Soybean Leaves and Stalks (SLS), Sugarcane Bagasse (SB), Tur Leaves (TL) and Wheat Straw (WS) were used as substrates. These untreated substrates were found to be well suited for the organism’s growth, producing good amounts of cellulases. It was observed that GPS, SB, TL, BP served as better substrates than others for fungal growth. These four substrates were thus selected for cellulase production study. They were supplemented with nitrogen source in the form peptone, casein, sodium nitrate and urea. This comparative study for the cellulase production via SSF indicated that cellulase activity produced by A. niger was higher in GPS supplemented with casein at pH 5 and incubation temperature of 50°C when incubated for 60 min.
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