Bovine Trichomonosis in Cattle: Trends in diagnostic approaches - Challenges and Opportunities
DOI:
https://doi.org/10.26438/ijsrbs.v12i1.669Keywords:
Venereal diseases, Culture methods, Point – of – care testing, ELISA, Lateral flow assayAbstract
Bovine Trichomonosis is a most neglected venereal diseases of cattle that causes severe reproductive failure. With current understanding, early disease detection and keeping diseased animals in confinement are the only viable strategies. There have also been several reports of zoonotic transmission in immunocompromised humans. According to World Organization of Animal Health recommendations, few culture and RT-PCR techniques for detecting Trichomonas foetus parasites in clinical samples have been established. Testing could not be expanded to regular screening and point-of-care settings due to a scarcity of testing kits, greater prices, and longer turnaround times. This article provides the trends in development of various diagnostic methods and their applicability. Furthermore this review highlights the limitations of the current methods and proposes the development of easy to use serological testing devices like Indirect ELISA (iELISA) and Lateral Flow Assay (LFA) rapid kit as Point of care testing (POCT) assays that are suitable for effective implementation of screening and monitoring of disease progress in herds.
References
Gookin, J. L., Breitschwerdt, E. B., Levy, M. G., Gager, R. B., &Benrud, J. G. “Diarrhea associated with Trichomonosis in cats”. Journal of the American Veterinary Medical Association, Vol. 215, Issue. 10, pp. 1450–1454,1999.
Collántes-Fernández, E., Fort, M.C., Ortega-Mora, L.M., Schares, G. . “Trichomonas” Parasitic Protozoa of Farm Animals and Pets. Springer, Cham.Vol.1, Issue.1, pp. 313 -388,2018.
BonDurant RH, Honigberg BM “Trichomonads of veterinary importance”. Parasitic protozoa. Academic, Vol.1, Issue.1, pp. 111–88, 1994.
Levy, M. G., Gookin, J. L., Poore, M., Birkenheuer, A. J., Dykstra, M. J., &Litaker, R. W.. “ Tritrichomonas foetus and not Pentatrichomonas hominis is the etiologic agent of feline trichomonal diarrhea”. The Journal of parasitology, Vol. 89, Issue. 1, pp. 99–104, 2003.
Van der Pol B. “Trichomonas vaginalis infection: the most prevalent nonviral sexually transmitted infection receives the least public health attention”. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, Vol. 44, Issue. 1, pp. 23–25, 2007.
Gookin, J. L., Stebbins, M. E., Hunt, E., Burlone, K., Fulton, M., Hochel, R., Talaat, M., Poore, M., & Levy, M. G.. “Prevalence of and risk factors for feline Tritrichomonas foetus and giardia infection”. Journal of clinical microbiology, Vol. 42, Issue 6, pp.2707–2710, 2004.
Yao, C., &Köster, L. S. “Tritrichomonas foetus infection, a cause of chronic diarrhea in the domestic cat”. Veterinary research, Vol.46, Issue. 1, pp. 35-39, 2015.
Morin-Adeline, V., Mueller, K., Conesa, A., &Šlapeta, J. “Comparative RNA-seq analysis of the Tritrichomonas foetus PIG30/1 isolate from pigs reveals close association with Tritrichomonas fetus BP-4 isolate 'bovine genotype”. Veterinary parasitology, Vol. 212, Issue. 4, pp.111–117, 2015.
Kleydman, Y., Yarlett, N., &Gorrell, T. E . “Production of ammonia by Tritrichomonas foetus and Trichomonas vaginalis.” Microbiology Reading, Vol. 150, Issue. 5, pp. 1139–1145, 2004.
Pereira-Neves, A., &Benchimol, M. “Tritrichomonas foetus: budding from multinucleated pseudocysts”. Protist, Vol. 160, Issue. 4 , pp.536–551, 2009.
BonDurant R. H. “Pathogenesis, diagnosis, and management of trichomoniasis in cattle. The Veterinary clinics of North America”. Food animal practice, Vol. 13, Issue. 2, pp.345–361, 1997.
Ondrak J. D. “Tritrichomonas foetus Prevention and Control in Cattle. The Veterinary clinics of North America”. Food animal practice, Vol. 32, Issue. 2, pp. 411–423, 2016.
Bondurant R. H . “Venereal diseases of cattle: natural history, diagnosis, and the role of vaccines in their control. The Veterinary clinics of North America.” Food animal practice, Vol. 21, Issue. 2, pp. 383–408. 2005.
Jin, Y., Schumaker, B., Logan, J., Yao, C.. “Risk factors associated with bovine trichomoniasis in beef cattle identified by a questionnaire”. Journal of medical microbiology, Vol. 63, Issue. 6, pp. 896–902. 2014.
Goodger, W. J., & Skirrow, S. Z. “Epidemiologic and economic analyses of an unusually long epizootic of Trichomoniasis in a large California dairy herd.” Journal of the American Veterinary Medical Association, Vol.189, Issue.7, pp.772–776, 1986.
Tolbert, M. K., & Gookin, J. L. “Mechanisms of Tritrichomonas foetus Pathogenicity in Cats with Insights from Venereal Trichomoniasis”. Journal of veterinary internal medicine, Vol. 30, Issue. 2, pp. 516–526, 2016.
Geethanjali Karli and KalaraniVarada. “BSPA-like and PMP-Like Proteins As A Potential Diagnostic Markers For Bovine Trichomoniasis.” Proceedings of National Conference on Impact of Biotechnology on Health Sector IBHS’20 Vol.1, pp. 52-58, 2020. ISBN Number: 978-93-5406-284-1 2020
Duboucher, C., Caby, S., Dufernez, F., Chabé, M., Gantois, N., Delgado-Viscogliosi, P., Billy, C., Barré, E., Torabi, E., Capron, M., Pierce, R. J., Dei-Cas, E., &Viscogliosi, E.. “Molecular identification of Tritrichomonasfoetus-like organisms as coinfecting agents of human Pneumocystis pneumonia.” Journal of clinical microbiology, Vol. 44, Issue. 3, pp. 1165–1168, 2006.
Okamoto, S., Wakui, M., Kobayashi, H., Sato, N., Ishida, A., Tanabe, M., Takeuchi, T., Fukushima, S., Yamada, T., & Ikeda, Y.. “Trichomonas foetus meningoencephalitis after allogeneic peripheral blood stem cell transplantation”. Bone marrow transplantation, Vol. 21, Issue.1, pp. 89–91,1998.
Yao C.. “Opportunistic human infections caused by Tritrichomonasspecies: a mini-review”. Clin. Microbiol. News, Vol. 34, Issue. 1, pp.127–131, 2012.
Zalonis, C. A., Pillay, A., Secor, W., Humburg, B., & Aber, R. . “Rare case of Trichomonal peritonitis. Emerging infectious diseases”, Vol. 17, Issue. 7, pp1312–1313, 2011.
Suzuki, J., Kobayashi, S., Osuka, H., Kawahata, D., Oishi, T., Sekiguchi, K., Hamada, A., & Iwata, S. “Characterization of a human isolate of Tritrichomonas foetus cattle/swine genotype infected by a zoonotic opportunistic infection”. The Journal of veterinary medical science, Vol. 78, Issue. 4, pp.633–640, 2016 .
Yao C. “Diagnosis of Tritrichomonas foetus-infected bulls, an ultimate approach to eradicate bovine trichomoniasis in US cattle.” Journal of medical microbiology, Vol. 62, Issue. 1 , pp1–9. 2013.
Yao C. “Control and eradication of Bovine Trichomonosis in Wyoming, USA by testing and culling positive bulls”. Veterinary research, Vol. 52, Issue.1, pp.129-135, 2021.
Dąbrowska, J., Karamon, J., Kochanowski, M., Sroka, J.,Zdybel, J., & Cencek, T.. “Tritrichomonas foetus as a Causative Agent of Tritrichomonosis in Different Animal Hosts”. Journal of veterinary research, Vol. 63, Issue. 4, pp.533–541. 2019.
McCool, C. J., Townsend, M. P., Wolfe, S. G., Simpson, M. A., Olm, T. C., Jayawardhana, G. A., & Carney, J. V..” Prevalence of bovine venereal disease in the Victoria River District of the Northern Territory: likely economic effects and practicable control measures.” Australian veterinary journal, Vol.65, Issue. 5, pp153–156, 1988.
Erasmus, J. A., De Wet, J. A., Van der Merwe, H. E., & Pienaar, G. C.. “Bovine Trichomoniasis in the northwestern Cape Province, western Transvaal and the Orange Free State”. Journal of the South African Veterinary Association, Vol. 60, Issue. 1, pp.51–52, 1989.
BonDurant, R. H., Anderson, M. L., Blanchard, P., Hird, D., Danaye-Elmi, C., Palmer, C., Sischo, W. M., Suther, D., Utterback, W., &Weigler, B. J. “Prevalence of trichomoniasis among California beef herds”. Journal of the American Veterinary Medical Association, Vol. 196, Issue. 10, pp1590–1593, 1990.
Pareek P., Sharma S., Bishnoi B., Gupta A. . “Trichomoniasis in cattle in North West Rajasthan.” Indian J AnimReprod. Vol. 15, Issue.1, pp.27–28, 1994.
Mancebo, O. A., Russo, A. M., Carabajal, L. L., &Monzon, C. M. . “Persistence of Tritrichomonas foetusin naturally infected cows and heifers in Argentina”. Veterinary parasitology, Vol. 59, Issue. 1, pp.7–11, 1995.
Gay, J. M., Ebel, E. D., & Kearley, W. P. “Commingled grazing as a risk factor for Trichomonosis in beef herds.” Journal of the American Veterinary Medical Association, Vol. 209, Issue. 3, pp. 643–646, 1996.
Rae, D. O., Crews, J. E., Greiner, E. C., & Donovan, G. A. “Epidemiology of Tritrichomonas foetus in beef bull populations in Florida”. Theriogenology, Vol.61, Issue. 4, pp. 605–618. 2004.
Mardones, F. O., Perez, A. M., Martínez, A., & Carpenter, T. E.. “Risk factors associated with Tritrichomonas foetus infection in beef herds in the Province of Buenos Aires, Argentina”. Veterinary parasitology, Vol. 153, Issue. 3-4, pp.231–237, 2008.
Yang, N., Cui, X., Qian, W., Yu, S., & Liu, Q. « Survey of nine abortifacient infectious agents in aborted bovine fetuses from dairy farms in Beijing, China, by PCR.” Acta veterinaria Hungarica, Vol.60, Issue. 1, pp. 83–92, 2012.
Mendoza-Ibarra, J. A., Pedraza-Díaz, S., García-Peña, F. J., Rojo-Montejo, S., Ruiz-Santa-Quiteria, J. A., San Miguel-Ibáñez, E., Navarro-Lozano, V., Ortega-Mora, L. M., Osoro, K., &Collantes-Fernandez, E. “High prevalence of Tritrichomonas foetus infection in Asturiana de la Montaña beef cattle kept in extensive conditions in Northern Spain.” Veterinary journal Vol. 193, Issue. 1, pp. 146–151, 2012.
Szonyi, B., Srinath, I., Schwartz, A., Clavijo, A., &Ivanek, R.. “Spatio-temporal epidemiology of Tritrichomonasfoetus infection in Texas bulls based on state-wide diagnostic laboratory data.” Veterinary parasitology, Vol.186, Issue. 3-4, pp. 450–455, 2012.
Yao C, Bardsley KD, Litzman EA, Hall ML, Davidson MR “Tritrichomonas foetus Infection in Beef Bull Populations in Wyoming”. J Bacteriol Parasitol. Vol. 2, Issue. 117, pp. 23-29, 2011
Guven, E., Bastem, Z., Avcioglu, H., &Erdem, H. “Molecular determination of Tritrichomonas spp. in aborted bovine foetuses in Eastern Anatolian Region of Turkey”. Veterinary parasitology, Vol. 196, Issue. 3-4, pp.278–282, 2013.
Molina, L., Perea, J., Meglia, G., Angón, E., & García, A.. “Spatial and temporal epidemiology of Bovine Trichomoniasis and bovine genital campylobacteriosis in La Pampa province Argentina”. Preventive veterinary medicine, Vol. 110, Issue. 3-4 , pp.388–394, 2013.
Islam, M., Talukder, M., Hossain, M., & Karim, M. “Investigation of sexually transmitted protozoan parasite Tritrichomonas foetus in cattle in Bangladesh.” Journal of the Bangladesh Agricultural University, Vol. 15, Issue 1, pp.47–54, 2017.
Mahajan., V, Banga H.,S, Gupta “Immunohistochemical and molecular approaches for the diagnosis of Trichomonas fetus in natural cases of bovine abortion”. Indian Journal of Veterinary Pathology, Vol. 41, Issue. 2, pp.116-118, 2017.
Filho, R., Malta, K. C., Borges, J. M., Oliveira, P., Filho, G., Nascimento, G. G., Mota, R. A., & Júnior, J. . “Prevalence and risk factors associated with Tritrichomonas foetus infection in cattle in the state of Paraíba, Brazil”. Acta parasitologica, Vol.63, Issue 2, pp.346–353, 2018.
Molina, L. L., Angón, E., García, A., Caballero-Villalobos, J., Giorgis, A. O., Moralejo, R. H., &Perea, J. “A retrospective epidemiological analysis of shared risk factors for bovine trichomoniasis and bovine genital campylobacteriosis in La Pampa province Argentina”. Preventive veterinary medicine, Vol.161, Issue. 1, pp. 109–114, 2018.
“Trichomoniasis”. OIE Terrestrial Manual. Vol. 3.4, Issue 15, pp.1210-1221, 2018.
Bryan L.A., Cambell J.R. & Gajadhar A.A. “ Effects of temperature on the survival of Tritrichomonas foetus in transport, Diamond’s and InPouch™ TF media”. Vet. Rec., Vol. 144, Issue. 1, pp.227 –232, 1999.
Lun Z.-R. & Gajadhar A.A. “A simple and rapid method for staining Tritrichomonas foetus and Trichomonas vaginalis.” J. Vet. Diagn. Invest., Vol. 11, Issue. 1, pp.471–474, 1999.
Amin, A., Liebhart, D., Weissenb.ck, H., & Hess, M.. “Experimental infection of turkeys and chickens with a clonal strain of Tetratrichomonas gallinarum induces a latent infection in the absence of clinical signs and lesions.” Journal of comparative pathology, Vol. 144, Issue. 1, pp. 55–62, 2011.
Skirrow S.Z. &Bondurrant R.H.. “Bovine Trichomoniasis.” Vet. Bull., Vol. 58, Issue. 1, pp.591–603, 1988.
Campero C.M., Rodriguez Dubra C., Bolondi A., Cacciato C., Cobo E., Perez S., Odeon A., Cipolla A. & Bondurant R.H.. “Two-step culture and PCR diagnostic approach for differentiation of non-T.foetus Trichomonads from genitalia of virgin bulls in Argentina”. Vet. Parasitol. Vol. 112, Issue.1, pp.167–175, 2003.
Casteriano A., Molini U., Kandjumbwa K., Khaiseb S., Frey C.F. &Šlapeta J. “Novel genotype of Tritrichomonas foetus from cattle in Southern Africa.” Parasitology, Vol.143, Issue. 1, pp.1954–1959, 2016.
Mcmillen L. & Lew A.E.. “Improved detection of Tritrichomonas foetus in bovine diagnostic specimens using a novel probe-based real time PCR assay”. Vet. Parasitol., Vol. 141, Issue.1, pp.204–215, 2006.
Felleisen R.S.J., Lambelet N., Bachmann P., Nicolet J., Muller N. & Gottstein B. “Detection of Tritrichomonas foetus by PCR and DNA enzyme immunoassay based on rRNA gene unit sequences”. J. Clin. Microbiol., Vol. 36, Issue. 1, pp.513–519, 1998.
Grahn, R. A., BonDurant, R. H., van Hoosear, K. A., Walker, R. L., & Lyons, L. A.. “An improved molecular assay for Tritrichomonas fetus”. Veterinary parasitology, Vol. 127, Issue. 1, pp. 33–41, 2005.
Oyhenart, J., Martínez, F., Ramírez, R., Fort, M., & Breccia, J. D.. “Loop mediated isothermal amplification of 5.8S rDNA for specific detection of Tritrichomonas fetus”. Veterinary parasitology, Vol. 193, Issue. 1-3, pp. 59–65, 2013.
Effinger L., Peddireddi L., Simunich M., Oberst R., O’connell C. & Leyva-Baca I. Pooling of cultured samples and comparison of multistate laboratory workflows with the MagMAX sample preparation system and VetMAX quantitative polymerase chain reaction reagents for detection of Tritrichomonas foetus-colonized bulls. J. Vet. Diagn. Invest., Vol.26, Issue 1, pp.72–87., 2014.
Pierce AE “The mucus agglutination test for the diagnosis of bovine trichomoniasis”. Vet Rec; Vol. 61, Issue. 1, pp.347–349, 1949.
BonDurant, R. H., van Hoosear, K. A., Corbeil, L. B., &Bernoco, D. “Serological response to in vitro-shed antigens of Tritrichomonas foetus in cattle”. Clinical and diagnostic laboratory immunology, Vol. 3, Issue. 4, pp.432–437, 1996.
Schaut, R. G., Corbeil, L. B., Blake, C. N., & Brewer, M. T. “Development of a bead-agglutination assay for rapid detection of Tritrichomonas fetus”. Veterinary parasitology, Vol.243, Issue. 1, pp.188–191. 2017.
Skirrow S.Z. &Bondurrant R.H.. Bovine Trichomoniasis. Vet. Bull., Vol.58, Issue. 1, pp.591–603, 1988.
Ikeda, J. S., BonDurant, R. H., & Corbeil, L. B. . “Bovine vaginal antibody responses to immunoaffinity-purified surface antigen of Tritrichomonas fetus”. Journal of clinical microbiology, Vol.33, Issue. 5, pp. 1158–1163,1995.
Rhyan, J. C., Wilson, K. L., Wagner, B., Anderson, M. L., BonDurant, R. H., Burgess, D. E., Mutwiri, G. K., & Corbeil, L. B. “Demonstration of Tritrichomonas foetus in the external genitalia and of specific antibodies in preputial secretions of naturally infected bulls”. Veterinary pathology, Vol. 36, Issue. 5, pp.406–411, 1999.
Cobo, E. R., Cano, D., Rossetti, O., & Campero, C. M. “Heifers immunized with whole-cell and membrane vaccines against Tritrichomonas foetus and naturally challenged with an infected bull”. Veterinary parasitology, Vol. 109, Issue. 3-4, pp.169–184,2002.
Cobo, E. R., Morsella, C., Cano, D., Cipolla, A., & Campero, C. M. “Immunization in heifers with dual vaccines containing Tritrichomonas foetus and Campylobacter fetus antigens using systemic and mucosalroutes.” Theriogenology, Vol.62, Issue. 8, pp.1367–1382, 2004.
Felleisen R. S. Comparative sequence analysis of 5.8S rRNA genes and internal transcribed spacer ITS regions of Trichomonad protozoa. Parasitology, Vol. 115, Issue. 2, pp.111–119, 1997.
Nickel, D. D., Olson, M. E., & Schultz, G. A. “An improved polymerase chain reaction assay for the detection of Tritrichomonas foetus in cattle. “ The Canadian veterinary journal = La revue veterinairecanadienne, Vol. 43, Issue. 3, pp.213–216, 2002.
Ho, M. S., Conrad, P. A., Conrad, P. J., LeFebvre, R. B., Perez, E., &BonDurant, R. H. Detection of bovine trichomoniasis with a specific DNA probe and PCR amplification system. Journal of clinical microbiology, Vol 32, Issue. 1, pp. 98–104, 1994.
Vashist S. K.. “Point-of-Care Diagnostics: Recent Advances and Trends”. Biosensors, Vol. 7, Issue. 4, pp. 62, 2017.
Manessis, Georgios &Gelasakis, Athanasios &Bossis, Yannis. “The challenge of introducing Point of Care Diagnostics in Farm Animal Health Management.” Biomedical Journal of Scientific & Technical Research. Vol. 14, Issue. 5, pp.10856-10859, 2019.
Abdel-Glil MY, Solle J, Wibberg D, Neubauer H, Sprague LD. “Chromosome-level genome assembly of Tritrichomonas foetus, the causative agent of Bovine Trichomoniasis” Sci Data. Vol. 20, Issue. 11(1), pp. 1030 – 1035, 2024. doi: 10.1038/s41597-024-03818-8
Hermadi HA, Khairullah AR, Damayanti Y, Safitri E, Tyasningsih W, Warsito SH, Fauzia KA, Wardhani BWK, Ekawasti F, Wibowo S, Fauziah I, Moses IB, Kurniasih DAA, Kusala MKJ, Julaeha J. “Bovine trichomoniasis: A hidden threat to reproductive efficiency”. Open Vet J. Vol. 14, Issue. 11, pp.2722-2730, 2024. doi: 10.5455/OVJ.2024.v14.i11.1.
Martin KA, Kovach K, Moscoso E, Carreiro E, Jesudoss Chelladurai JRJ, Brewer MT. “Assessment of in vitro efficacy for common surface disinfectants and antiseptics against Tritrichomonas foetus trophozoites”. Front Vet Sci. Vol. 9, Issue. 10, pp. 128- 138, 2023. doi: 10.3389/fvets.2023.1282274.
}. Viola MN, Elías IC, Signorini M, Molineri AI, Russo AM, Zimmer PA, Lozina LA, Gimenez JN. “Prevalence and geographical distribution of bovine sexually transmitted diseases in the province of Formosa, Argentina”. Rev Argent Microbiol. Vol. 56, Issue. 2, pp.147-152, 2024. doi: 10.1016/j.ram.2023.07.004.
Santos JHM, Boe-Hansen GB, Siddle HV, Nguyen LT, Raza A, McGowan M, McCosker KD, Tabor AE. “Systematic Review of Vaccine Strategies Against Tritrichomonas foetus Infection in Cattle: Insights, Challenges, and Prospects”. Parasite Immunol. Volume 4, Issue 1, pp.234 -240, 2025. doi: 10.1111/pim.70003.
Ruqaya Muther Jalil Ewadh, "Nano Biotechnology, Usage, Application and Challenges in Microbiology (Mini Review)," International Journal of Scientific Research in Biological Sciences, Vol.11, Issue.3, pp.34-39, 2024.
Durgha Sakthi R.S., Sindhu R., Prabu D., Rajmohan M., Bharathwaj V.V., Dinesh Dhamodhar M., Sathiyapriya, "Comparing Biocompatibility and Efficacy of Hydroxyapatite Nanocomposites at Nano-level for its Biomedical Application: A Systematic Review," International Journal of Scientific Research in Biological Sciences, Vol.10, Issue.1, pp.6-11, 2023.
Manessis, G., Gelasakis, A. I., &Bossis, I. “Point-of-Care Diagnostics for Farm Animal Diseases: From Biosensors to Integrated Lab-on-Chip Devices”. Biosensors, Volume 12, Issue. 7, pp.455-478, 2022.
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