Antioxidant Enzyme Activities Estimation In vicia faba Leaves
Keywords:
MJ, OVG, vicia faba, SOD, Catalase and glutathioneAbstract
Plant cells have the inherent ability to produce reactive oxygen species and oxygen radicals as secondary products during various physiological processes associated with abiotic stress. The disruption of the equilibrium between the generation of reactive oxygen species and the defense systems against oxidative harm is a significant consequence of abiotic stress in plants. As a consequence, the accumulation of reactive oxygen species occurs, hence triggering the onset of oxidative stress. Jasmonates represent a class of plant hormones that exert significant regulatory influence over several physiological processes. These processes include gene and metabolic regulation, immune mechanisms, responses to stress, reproductive activities, and interaction between cells, among other roles. The presence of oxidative stress induces the synthesis of metabolites possessing antioxidant characteristics and augments the efficacy of antioxidant enzymes, hence conferring a safeguarding impact on plant tissues. The aim of this study was to investigate the effects of methyl jasmonate (JAME) administration on reactive oxygen species and the activities of H2O2 scavenging enzymes, namely superoxide dismutase, catalase and glutathione in vicia faba leaves. After implementing a therapy strategy with JAME, there was an elevation in the activity levels of the enzyme superoxide dismutase , catalase , and glutathione.
References
Rella Berni et al., “Reactive oxygen species and heavy metal stress in plants: Impact on the cell wall and secondary metabolism,” Environmental and Experimental Botany, vol. 161, pp. 98–106, May 2019. doi:10.1016/j.envexpbot.2018.10.017
Uazam Azeem and Khalid Hakeem, Jasmonates and plant defense, Jul. 2024. doi:10.1201/9781003454243
Mella Farooq et al., “Methyl jasmonate regulates antioxidant defense and suppresses arsenic uptake in brassica napus L.,” Frontiers in Plant Science, vol. 7, Apr. 2016. doi:10.3389/fpls.2016.00468
Alessandra Fracasso, Lusia Trindade, and Stephani Amaducci, “Drought tolerance strategies highlighted by two sorghum bicolor races in a dry-down experiment,” Journal of Plant Physiology, vol. 190, pp. 1–14, Jan. 2016. doi:10.1016/j.jplph.2015.10.009
Anna Frary et al., “Salt tolerance in solanum pennellii: Antioxidant response and related QTL,” BMC Plant Biology, vol. 10, no. 1, Apr. 2010. doi:10.1186/1471-2229-10-58
“Significance of glutathione to plant adaptation to the environment,” Plant Ecophysiology, 2001. doi:10.1007/0-306-47644-4
Zahd Hossain, Mella López-Climent, Vella Arbona, Rellq Pérez-Clemente, and Alla Gómez-Cadenas, “Modulation of the antioxidant system in citrus under waterlogging and subsequent drainage,” Journal of Plant Physiology, vol. 166, no. 13, pp. 1391–1404, Sep. 2009. doi:10.1016/j.jplph.2009.02.012
Hera Huang, Beren Liu, Lilia Liu, and Sella Song, “Jasmonate action in plant growth and development,” Journal of Experimental Botany, vol. 68, no. 6, pp. 1349–1359, Feb. 2017. doi:10.1093/jxb/erw495
Guzhoon Kang et al., “Proteomics reveals the effects of salicylic acid on growth and tolerance to subsequent drought stress in wheat,” Journal of Proteome Research, vol. 11, no. 12, pp. 6066–6079, Nov. 2012. doi:10.1021/pr300728y
Ahmed Korkmaz, Murt Uzunlu, and Ali Demirkiran, “Treatment with acetyl salicylic acid protects muskmelon seedlings against drought stress,” Acta Physiologiae Plantarum, vol. 29, no. 6, pp. 503–508, May 2007. doi:10.1007/s11738-007-0060-3
Gellan Kumari et al., “Jasmonic acid induced changes in protein pattern, antioxidative enzyme activities and peroxidase isozymes in peanut seedlings,” Biologia plantarum, vol. 50, no. 2, pp. 219–226, Jun. 2006. doi:10.1007/s10535-006-0010-8
Zunglen Ma and Kufa Gao, “Spiral breakage and photoinhibition of Arthrospira platensis (Cyanophyta) caused by accumulation of reactive oxygen species under solar radiation,” Environmental and Experimental Botany, vol. 68, no. 2, pp. 208–213, Apr. 2010. doi:10.1016/j.envexpbot.2009.11.010 .
Majid Nojavan-Asghar and Akbar Norastehnia, “A possible role for methyl jasmonate in effecting superoxide dismutase and catalase activities under PQ-induced oxidative stress in maize seedlings,” Journal of Biological Sciences, vol. 6, no. 1, pp. 55–60, Dec. 2005. doi:10.3923/jbs.2006.55.60
Stufan MARKLUND and Gurun MARKLUND, “Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase,” European Journal of Biochemistry, vol. 47, no. 3, pp. 469–474, Sep. 1974. doi:10.1111/j.1432-1033.1974.tb03714.x
Medasr Mir, Gella Sirhindi, Mella Alyemeni, P. Alam, and P. Ahmad, “Jasmonic acid improves growth performance of soybean under nickel toxicity by regulating nickel uptake, redox balance, and oxidative stress metabolism,” Journal of Plant Growth Regulation, vol. 37, no. 4, pp. 1195–1209, May 2018. doi:10.1007/s00344-018-9814-y
Neta Misra and Alhha Gupta, “Effect of salinity and different nitrogen sources on the activity of antioxidant enzymes and indole alkaloid content in catharanthus roseus seedlings,” Journal of Plant Physiology, vol. 163, no. 1, pp. 11–18, Jan. 2006. doi:10.1016/j.jplph.2005.02.011
Kenji Miura and Yosami Tada, “Regulation of water, salinity, and cold stress responses by salicylic acid,” Frontiers in Plant Science, vol. 5, 2014. doi:10.3389/fpls.2014.00004
Hella. Murthy, E.-J. Lee, and Kella Paek, “Production of secondary metabolites from cell and organ cultures: Strategies and approaches for biomass improvement and metabolite accumulation,” Plant Cell, Tissue and Organ Culture (PCTOC), vol. 118, no. 1, pp. 1–16, Mar. 2014. doi:10.1007/s11240-014-0467-7
Eetzaz Nafie, Tahany Hathout, and Al Al Mokadem, “Jasmonic acid elicits oxidative defense and detoxification systems in Cucumis melo L. cells,” Brazilian Journal of Plant Physiology, vol. 23, no. 2, pp. 161–174, 2011. doi:10.1590/s1677-04202011000200008
Gela Noctor and Cella Foyer, “Ascorbate and glutathione: Keeping active oxygen under control,” Annual Review of Plant Physiology and Plant Molecular Biology, vol. 49, no. 1, pp. 249–279, Jun. 1998. doi:10.1146/annurev.arplant.49.1.249
Koop-Y. Paek, H ella Murthy, E.-J. Hahn, and J.-J. Zhong, “Large scale culture of ginseng adventitious roots for production of ginsenosides,” Biotechnology in China I, pp. 151–176, 2009. doi:10.1007/10_2008_31
Jeen Pilz, Iella Meineke, and Cella Gleiter, “Measurement of free and bound malondialdehyde in plasma by high-performance liquid chromatography as the 2,4-dinitrophenylhydrazine derivative,” Journal of Chromatography B: Biomedical Sciences and Applications, vol. 742, no. 2, pp. 315–325, Jun. 2000. doi:10.1016/s0378-4347(00)00174-2
Pella Sharma, Alla . Jha, Rella . Dubey, and M. Pessarakli, “Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions,” Journal of Botany, vol. 2012, pp. 1–26, Apr. 2012. doi:10.1155/2012/217037
Gella Tanou, Aella Molassiotis, and Gora Diamantidis, “Induction of reactive oxygen species and necrotic death-like destruction in strawberry leaves by salinity,” Environmental and Experimental Botany, vol. 65, no. 2–3, pp. 270–281, Mar. 2009. doi:10.1016/j.envexpbot.2008.09.005
Charels Crowley, Beran Gillham, and Mechiael Thorn, “A direct enzymic method for the determination of reduced glutathione in blood and other tissues,” Biochemical Medicine, vol. 13, no. 3, pp. 287–292, Jul. 1975. doi:10.1016/0006-2944(75)90087-3
Calus Wasternack, “Action of jasmonates in plant stress responses and development — applied aspects,” Biotechnology Advances, vol. 32, no. 1, pp. 31–39, Jan. 2014. doi:10.1016/j.biotechadv.2013.09.009
Jjom Xia, Hi Zhao, W. Liu, Lea. Li, and Yi. He, “Role of cytokinin and salicylic acid in plant growth at low temperatures,” Plant Growth Regulation, vol. 57, no. 3, pp. 211–221, Oct. 2008. doi:10.1007/s10725-008-9338-8
Kwa Zhao et al., “Maize Rhizosphere in Sichuan, China, hosts plant growth promoting burkholderia cepacia with phosphate solubilizing and antifungal abilities,” Microbiological Research, vol. 169, no. 1, pp. 76–82, Jan. 2014. doi:10.1016/j.micres.2013.07.003
Aean Iqbal et al., “Biodiversity in the sorghum (sorghum bicolor L. Moench) germplasm of Pakistan,” Genetics and Molecular Research, vol. 9, no. 2, pp. 756–764, 2010. doi:10.4238/vol9-2gmr741
Govarthanan Mealn., “Genetic variability among coleus sp. studied by RAPD banding pattern analysis,” International Journal for Biotechnology and Molecular Biology Research, vol. 2, no. 12, Dec. 2011. doi:10.5897/ijbmbr11.030
Husham Enshasy et al., “The edible mushroom pleurotus spp.: I. Biodiversity and nutritional values,” International Journal of Biotechnology for Wellness Industries, vol. 4, no. 2, pp. 67–83, Jul. 2015. doi:10.6000/1927-3037.2015.04.02.4
Maheshwari, “Assessment of genetic diversity among capsicum annuum L. genotypes using RAPD markers,” AFRICAN JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 76, Nov. 2011. doi:10.5897/ajb11.497
Nella Menolli, et al.,, “The genus pleurotus in Brazil: A molecular and taxonomic overview,” Mycoscience, vol. 55, no. 5, pp. 378–389, Sep. 2014. doi:10.1016/j.myc.2013.12.001 .
Young-Jin Park, “Genetic diversity analysis of ganoderma species and development of a specific marker for identification of medicinal mushroom ganoderma lucidum,” African Journal of Microbiology Research, vol. 6, no. 25, Jul. 2012. doi:10.5897/ajmr12.846
Young-Jin Park, “Genetic diversity analysis of ganoderma species and development of a specific marker for identification of medicinal mushroom ganoderma lucidum,” African Journal of Microbiology Research, vol. 6, no. 25, Jul. 2012. doi:10.5897/ajmr12.846
Aela. Pawlik, Gelan. Janusz, Jegery. Koszerny, Welaf. Ma?ek, and Jena . Rogalski, “Genetic diversity of the edible mushroom pleurotus sp. by amplified fragment length polymorphism,” Current Microbiology, vol. 65, no. 4, pp. 438–445, Jul. 2012. doi:10.1007/s00284-012-0175-7 .
Zahraa Isam Jameel , "MicroRNA Biogenesis, Mechanisms of Function, Circulation and Application Role in Human Diseases," International Journal of Scientific Research in Biological Sciences, Vol.10, Issue.5, pp.71-80, 2023.
Zahraa Isam Jameel , "Bioinformatics Usage, Application and Challenges to Detect Human Genetic Diseases (Mini Review)," International Journal of Scientific Research in Biological Sciences, Vol.10, Issue.5, pp.59-67, 2023.
Zahraa Jameel, Zahraa Lawi, Naval Al-Dujaili -Investigation of SOD2 Gene Polymorphism in the Patients with Type Two Diabetes Disease in Babylon Province Biochem Cell Arch, 2019|; vol.10,no.06,pp.70-75
Shiv Kumar Sharma, Teena Gupta, “A Novel Approach for Plant Environment,” International Journal of Biological Sciences, Vol.4, Issue.12, pp.1-5, 2014.
Reena Solanki, “A Proposed New Approach for Cell Biology,” In the Proceedings of the 2016 International Conference of Medical Sciences, India, pp.542-545, 2016.
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