Microscopic and Molecular Diagnosis of Lice infesting Buffaloes in Babylon Province, Iraq
Keywords:
Lice, Buffaloes, PCR, DNA sequencing,, IraqAbstract
Lice are harmful ectoparasites that feed on human and animal blood and are vital vectors for various parasitic diseases in mammals. Current data shows that the lice-infesting buffaloes in Babylon city were not identified. Thus, the present study was done to isolate and identify lice species infesting buffaloes using microscopic and molecular techniques. The results of clinical inspection revealed that 5 out of 1000 animals (%0.5) were only infested between September 2023 and January 2024. The microscopic examination of isolated lice identified one genus, Haematopinus. The distribution of lice in buffaloes according to sex in females was higher than in males, which was 0.62% compared to male 0.28%. The rate of lice infection according to age was high in the 11-18 years of age with a percentage of 0.83% and the lowest rate in the 4-10 years of age with a percentage of 0.37%. Furthermore, the lice infestation rates according to the area recorded that Awfi had the highest infestation rate of 2.22%, followed by the districts of Al-Qasim and Al-Sadda, which were 1.66% and 1.33%, respectively. The molecular study, using PCR and sequencing techniques, confirmed microscopic findings, where all isolated lice were under Haematopinus tuberculatus. In conclusion, according to currently available data, the present study is believed to be the first molecular study for the identification of lice species infesting buffaloes in Babylon province, Iraq.
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