In-Vitro Total Tocopherols, Phenol and Anti-oxidative Levels of Seeds’ oil of Telfairia occidentalis
Keywords:
Fluted pumpkin, total phenol, nitric acid, tocopherol, fluted pumpkinAbstract
The seeds of fluted pumpkins are usually eaten boiled or pulverized and taken as food additive locally. As a result of this manner of utilization by the locals, this study was conducted to ascertain the contents of the total tocopherol, phenolic and the antioxidative property present in the oil of the seed. The pods of the fluted pumpkin were gotten from a farm within the Benin metropolis, Nigeria. The seeds were ground in a blender and afterward exposed to soxhlet extraction. Other standard methods were employed in this study. The quantitative analysis of these parameters showed Total Tocopherols (76.0 mg/kg ± 3.11), Total phenolic (3.34 mg of garlic acid equivalent ± 0.15). Percentage inhibition of DPPH was recorded to be 47.95 % ± 3.47, Nitric oxide scavenging capacity was 56.47 % ± 2.37 and ABTS was 51.06 % ± 2.60 respectively. The findings revealed that the seed oil of T. occidentalis possessed beneficial antioxidant properties which could be harnessed in management of disease like diabetes and cancer among others.
References
M.O. Eke, J.O. Elechi, “Food Safety and Quality Evaluation of Street Vended Meat Pies Sold in Lafia Metropolis, Nasarawa State, Nigeria”, International Journal of Scientific Research in Biological Science, Vol.8, Issue.1, pp.88-98, 2021.
B.C Makowa, “Determination of Water in Some Dehydrated Foods in Advance Chemistry: Series 3,” Am. Chem. Soc., Washington D.C. pp.25-27, 1950.
Adewuyi, R.A. Oderinde, “Fatty acid composition and lipid profile of Diospyros mespiliformis, Albizia lebbeck and Caesalpinia pulcherrima seed oil from Nigeria”, International Journal of Food Science, Vol.2014, pp.1-6, 2014.
M. Akoroda. Ethnobotany of Telfairiaoccidentalis (Cucurbitaceae) among Igbos of Nigeria, Econ. Bot., Vol.44, Issue 1, pp.29-39, 1994
E.S. Oluwole, A.O. Falode, O.O. Ogundipe, “Anti-Inflammatory Effect of Some Common Nigerian Vegetables”, Nigerian Journal of Physiological Sciences, Vol.18, pp.35-38, 2003.
T.C. Ajayi, E.U. Omakaro, N.K.D. Halin, “Erythropoietic values of pumpkin leave extract Telfairiaoccidentalis in rabbits a prelimary study”, Nig. J. Physiol. Sciences, Vol.16, pp.1-3, 2000.
O. Eseyin, A.C. Igboasoiyi, E. Oforah, P. Chin, B. Okoli, “Effects Of Extracts of Telfairiaoccidentalis Leaves On Some Biochemical Parameters In Rat”, Global Journal of Pure and Applied Sciences, Vol.1, Issue1, pp.85-87, 2005.
O.A. Eseyin, P. Ebong, U.E. Eyong, E. Umo, E. Attih, “Comparative hypoglycaemic effects of ethanolic and aqueous extracts of the leaf and seed of Telfairia occidentalis” Turk. J. Pharm. Sci., Vol.7, Issue 1, pp.29-34, 2010.
Al-Salman F, Ali Redha A., Al-Zaimoor Z, “Inorganic Analysis and Antioxidant Activity of Shilajit”, International Journal of Scientific Research in Chemical Sciences, Vol. 7, Issue. 3, pp. 05- 10, 2020
A. Aderibigbe, B. Lawal, J. Oluwagbemi, “The antihyperglycaemic effect of Telfairia occidentalis in mice”, Afr. J. Med. Med. Sci., Vol.28, pp.115 -171, 1999
S.O. Nwozo, T.L. Adebowale, B.E. Oyinloye, “Defatted Detarium senegalense seed-based diet alters lipid profile, antioxidants level and sperm morphology in male albino rats”, Int. J. Biol. Chem. Sci., Vol10, Issue 3, pp.928-943, 2016.
I.O.O. Aiyelaagbe, A,A, Kintomo, “Nitrogen Response of Fluted Pumpkin (Telfairiaoccidentalis Hook.F) Grown Sole or Intercropped with Banana. Nutrient Cycling in Agroecosystems”, Vol.64, pp.231-235, 2002.
E. Nwanna, G. Oboh, “Antioxidant and Hepatoprotective Properties of Polyphenol Extracts from Telfairia occidentalis (Fluted Pumpkin) leaves on acetaminophen induced liver damage”, Pak. J. Biol. Sci.,Vol.10, Issue 16, pp.2682-2687, 2007.
S.Y. Giami, M.N. Adindu, M.O. Akusu, J.N. Emelike, “Compositional, functional and storage properties of flours from raw and heat processed African breadfruit (Treculiaafricana Decne) seeds”, Plant foods for human nutrition (Dordrecht, Netherlands), Vol. 55, Issue 4, pp.357-368, 2000.
T.N. Fagbemi, A. Oshodi, “Chemical composition and functional properties of full-fat fluted pumpkin seed flour (Telfairia occidentalis)”, Nigerian Food Journal, Vol.9, pp.26-32, 1991.
E.G. Bligh, W.J. Dyer, “A rapid method of total lipid extraction and purification”, Can. J. Biochem. Physiol., Vol. 37, pp. 911-917, 1959.
AOCS. A.O.C.S, “Official method ce 8-89 (reapproved 1997) determination of tocopherols and tocotrienols in vegetable oils and fats by HPLC”, 5 pp. In: Official Methods and Recommended Practices of the American Oil Chemists` Society 5th edition. American Oil Chemists` Society, Champaign, IL., 1997.
X. Dewanto, K. Wu, K. Adom, R.H. Liu. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity, J. Agric. Food Chem. Vol. 50, pp. 3010–3014, 2002.
Al-Salman F, Ali Redha A., Al-Zaimoor Z, “Inorganic Analysis and Antioxidant Activity of Shilajit”, International Journal of Scientific Research in Chemical Sciences, Vol. 7, Issue. 3, pp. 5- 10, 2020.
D.C. Garrat, “The Quantitative Analysis of Drugs”, 3rd Edn. Chapman and Hall, Japan, pp.456-458, 1964.
R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, “Rice-Evans C. Antioxidant activity: applying an improved ABTS radical cation decolorization assay”, Free Radical Biol. Med., Vol.26, pp.1231-1237, 1999.
D. Pericin, V. Krimer, S. Trtvic, L. Radulovic, “The Distribution of Phenolic Acids in Pumpkin’s Hull-Less Seed, Skin, Oil Cake Meal, Dehulled Kernel and Hull”, Food Chem., Vol.113, pp.450–456, 2009.
A. Siger, M. Nogala-Kalucka, E. LampartSzczapa, “The Content and Antioxidant Activity of Phenolic Compounds on Cold-Pressed Plant Oils”, J. Food Lipids,Vol.15, pp.137–149, 2008.
M. Antolovich, P.D. Prenzler, E. Patsalides, S. Mcdonald, K. Robards, “Methods for testing antioxidant activity”, Analyst., Vol.127, pp.183–198, 2002.
D. Boskou, “Sources of Natural Phenolic Antioxidants”, Trends Food Sci. Tech., Vol.17, pp.505–512, 2006.
H. Yoshida, Y. Tomiyama, Y. Hirakawa, Y. Mizushina, “Microwave roasting effects on the oxidative stability of oils and molecular species of triacylglycerols in the kernels of pumpkin (Cucurbita spp.) seeds”, J. Food Compos. Anal.,Vol.19, Issue 4, pp.330-339, 2006.
J. Tsaknis, S. Lalas, E.S. Lazos, “Characterization of Crude and Purified Pumpkin Seed Oil”, Gras. Aceit., Vol.48, pp.267-272, 1997.
S.N. Nakic D. Rade, D. Skevin, D. Strucelj, Z. Mokrovcak, M. Bartolic, “Chemical Characteristics of Oils from Naked and Husk Seeds of Cucurbita pepo L”, Eur. J. Lipid Sci. Technol., Vol.108, pp.936–943, 2006.
T. Nakagawa, T. Yokozawa, “Direct scavenging of nitric oxide and superoxide by green tea”, Food Chem. Toxicol., Vol.40, pp.1745–1750, 2002.
S. Aghaie, H. Nikzad, J.A. Mahabadi, M. Taghizadeh, A. Azami-Tameh, A. Taherian, S.M. Sajjadian, M. Kamani, “Protective effect of combined pumpkin seed and ginger extracts on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with cyclophosphamide”, Anat. Sci. Int., Vol.91, pp.382–390, 2015.
Downloads
Published
How to Cite
Issue
Section
License

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors contributing to this journal agree to publish their articles under the Creative Commons Attribution 4.0 International License, allowing third parties to share their work (copy, distribute, transmit) and to adapt it, under the condition that the authors are given credit and that in the event of reuse or distribution, the terms of this license are made clear.