Impact of Oocytes with Morphological Abnormalities on Blastocyst Formation Rate
Keywords:
oocyte abnormalities, ICSI, Blastocyst rate, intracytoplasmic sperm injectionAbstract
Objective: Aim of the study is to evaluate the intracytoplasmic and extracytoplasmic abnormalities of the human oocytes and their influence on potential embryo development and blastocyst formation rate outcome. Exclusive criteria: Cycles from cryopreserved oocytes and the patient underwent agonist stimulation protocol were excluded, Severe male factor infertility (oligozoospermia<10 million/ml)Oocyte morphology was evaluated at the time of ICSI and scored according to the scoring system described by Martin-Wilding et al. The inseminated oocytes were cultured according to standard protocol. Fertilization was assessed at 17-18 hours and cleavage was assessed at 26 hours after insemination respectively. Embryo grading was done on day 5 using Gardner’s embryo grading system. Material and Methods: A Single center, prospective observational study with patients undergoing intracytoplasmic sperm injection (ICSI) treatment between September 2020 to January 2021 was included. Oocytes are graded according to their maturity for all oocytes and subsequently assessed for fertilization, cleavage, and blastocyst grading. Correlation between oocyte morphology and fertilization rate, cleavage rate, and blastocyst rate assessed using appropriate statistical method using (SPSS) version 16.0. Result: A total of 50 patients and oocytes were studied, in that 371 oocytes underwent intracytoplasmic sperm injection (ICSI) with fertilization rate 78.4 %(291/371), cleavage rate 81%(236/291), and blastocyst rate 66.5%(157/236). Embryo grades were A-37.5%, B-50.9 %, and C-11.% oocyte morphology was not predictive of fertilization, cleavage, and blastocyst rates. Conclusion: There is no statically difference between blastocyst formation rate between morphologically normal oocytes and oocytes with morphological abnormalities like CLGC, SER, and Refractile body.
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