In vitro Callus Induction and Preliminary Phytochemical Studies of Cissampelos pareira L
Keywords:
Cissampelos pareira, callus, growth regulators, phytochemical screeningAbstract
The present investigation was aimed to evaluate the effect of growth regulators on induction of callus from leaf segments of Cissampelos pareira. The surface sterilized young leaves were inoculated on Murashige and Skoog (MS) medium fortified with different concentration and combination of growth regulators for induction of callus. The phytochemical constituents were analyzed in the different solvent extracts using standard methods. Maximum callus induction was observed on MS medium supplemented with 2 mg/L of NAA or 2, 4-D alone and in combinations of NAA (2 mg/L) + BAP (1 mg/L) and NAA (2 mg/L) + KIN (0.5 mg/L). The preliminary phytochemical screening of leaf and leaf derived callus revealed the presence of flavonoids, alkaloids, phenols, terpenoids, coumarins etc. The developed protocol is useful for large scale production of callus from leaf explants which may be useful for isolation of important bioactive compounds for the treatment of various diseases.
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