Anti-biofilm Potential of Biosurfactant Isolated from Lactobacillus Spp. Against Biofilms of Candida Spp.
Keywords:
Biosurfactant, Candida spp., Lactobacillus spp., Pre-coating methodAbstract
Work is focused to study the anti-biofilm activities possessed by various strains of Lactobacillus spp. over pathogenic Candida spp. Liquid-liquid extraction of the centrifuged Lactobacillus spp. in an equiratio of supernatant and ethyl acetate followed by evaporation of the solvent gives crude form of the biosurfactant. Pre-coating of the Candida spp. samples measure the percentage adhesion on the walls of 96-well microtiter plates at four different concentrations (0.05, 0.1, 0.125 and 0.2 g/ml) of the extracted biosurfactant. Findings reveal that the isolates inhibit (~75%) the adhesion of Candida cells. Conducting the pre-coating method at 0.2 g/mL of crude biosurfactant of the 10-5Cb1 isolate reduced (~93%) the cell adhesion of Candida spp. However, few of the Lactobacillus isolates reduced (~90%) adhesion of Candida cells.
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