A Flow Virometry Process for Detection of SARS-Cov-2 Proposed for Large-Scale Screening of COVID-19 Cases

Authors

  • Niraja Soni Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd., Navi Mumbai, India
  • Puja Pai Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd., Navi Mumbai, India
  • Raja Krishna Kumar G. Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd., Navi Mumbai, India
  • Venkatesh Prasad Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd., Navi Mumbai, India
  • Santanu Dasgupta Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd., Navi Mumbai, India
  • Bhaskar Bhadra Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd., Navi Mumbai, India

Keywords:

COVID-19, Severe acute respiratory syndrome (SARS), SAR-CoV-2, flow Cytometry

Abstract

The coronavirus disease, COVID 19, caused by SARS-CoV-2 has spread rapidly over 210 countries and declared as pandemic by World Health Organization (WHO). WHO also emphasized on scaling-up testing capacity, followed by isolation of infected individuals, and contact tracing, as the "backbone" of managing the pandemic. Globally, the detection of SARS-CoV-2 in patients are done by RT-PCR and blood antibody-based testing. However, immediate expansion of the scale of screening is very important to cover major fraction of 1.3 billion population of India. We, therefore, propose a flow-cytometry based high-throughput screening systems for testing of COVID 19 cases. The swab-samples in viral transport media could be analyzed using ?indirect flow cytometry?, where virus particle binds to specific primary antibody and the resulted virus-antibody complex then binds to a fluorescent tagged secondary antibodies. The fluorescence signal can distinguish on a flow-channel to identify viral load in a test sample. In the present article, we have summarized a flow-virometry process for detection of various viruses and have proposed possible application of the process in screening of COVID 19.

 

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Published

2020-06-30

How to Cite

[1]
N. Soni, P. Pai, R. K. Kumar G., V. Prasad, S. Dasgupta, and B. Bhadra, “A Flow Virometry Process for Detection of SARS-Cov-2 Proposed for Large-Scale Screening of COVID-19 Cases”, Int. J. Sci. Res. Biol. Sci., vol. 7, no. 3, pp. 85–91, Jun. 2020.

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Vol. 7 No. 3 (2020): June Edition

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Research Article

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