Enhanced Production of Therapeutically Valuable Withaferin A From In Vitro Propagated Withania Somnifera (L.) Dunal
Keywords:
Withania somnifera, callus, regeneration, micropropagation, withaferin A, HPLCAbstract
Withanolides from Withania somnifera are one amongst the most exploited secondary metabolites for their enormous therapeutical and pharmaceutical benefits. Large scale production of these herbs from different explants of in vitro germinated seedlings were tried in order to meet the growing market demand. A rapid and efficient indirect regeneration protocol was optimized from the leaf explants of W.somnifera. Compact green calli were obtained from leaf explants cultured on MS media supplemented with 2mg/L BAP showed good regeneration potential. Maximum multiple shoots were obtained from calli grown on MS media fortified with 1 mg/L BAP and 0.1 mg/L NAA. The micro shoots were excised and efficient rooting was induced in MS media supplemented with 0.2 mg/L IBA. The in vitro propagated plantlets were found to be high in their withaferin A content (3.21mg/g DW). The withaferin A content was around 2.12-fold higher than the control explants. Micropropagation of such a highly valuable medicinal plant is extremely necessary in meeting the growing demands of this therapeutically valuable medicinal plant.
References
S.K. Sharma, L. Gupta, “A Novel Approach for Cloud Environment,” International Journal of Scientific Research in Biological Sciences, Vol.4, Issue.12, pp.1-5, 2014.
V.K.Gauttam, A.N. Kalia, “Development of polyherbal antidiabetic formulation encapsulated in the phospholipids vesicle system” J Adv Pharm Technol Res. Vol. 2, pp.108-117, 2013.
A. Rani, M. Kumar, S. Kumar, “Effect of phytohormones on shoot apex and leaf explants of Withania somnifera (Ashwagandha)” J Applied and Natural Sci. Vol. 8, Issue.1, pp. 412-415, 2016.
H.Patel, R. Krishnamurthy, “ Elicitors in Plant Tissue Culture” J Pharmacogn Phytochem. Vol.2, pp. 60-65,2017.
G. Sivanandhan, M. Arun, S. Mayavan et al., “Optimization of elicitation conditions with methyl jasmonate and salicylic acid to improve the productivity of withanolides in the adventious root cultures of Withania somnifera (L.) dunal” Applied Biochemisry and Biotechnology. Vol. 2, Issue. 1, pp. 681-696, 2012.
G. Sivanandhan, M. Arun, S.Mayavan et al., “Chitosan enhances withanolides production in adventitious root cultures of Withania somnifera (L.) Dunal” Industrial Crops and Products. Vol.37, Issue.1, pp. 124-129, 2012a.
G. Sivanandhan, G.K. Dev, J. Theboral, N. Selvaraj, A. et al., “Sonication, vacuum infiltration and thiol compounds enhance the Agrobacterium -mediated transformation frequency of Withania somnifera (L.) Dunal” Plos One. Vol.10, Issue. 4, 2015.
T. Murashige, F. Skoog, F “A revised medium for rapid growth and bio assays with Tobacco Tissue Cultures” Physiologia Plantarum. Vol.15. pp.473-497, 1962.
O.L. Gamborg, R.A. Miller, K. Ojima “Nutrient requirements of suspension cultures of soybean root cells” Experimental Cell Research. Vol.50, Issue.1, pp. 151-158, 1968.
R.U. Schenk, A.C. Hildebrandt, “Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures” Canadian Journal of Botany. Vol. 50, Issue.1, pp. 199-204, 1972.
J.P. Nitsch, J.P , C “Nitsch Haploid plants from pollen grains” Science. Vol. 163, Issue. 3862, pp. 85-87, 1969.
P.K.Khanna, A. Kumar, R. Chandra, V. Verma, “Germination behaviour of seeds of Withania somnifera (L.) Dunal: A high value medicinal plant” Physiology and Molecular Biology of Plants. Vol. 19, Issue.3, pp. 449-454, 2013.
I. Sivanesan , K. Murugesan, “An efficient regeneration from nodal explants of W. somnifera Dunal” Asian Journal of Plant Sciences. Vol.7, Issue.3, pp. 551-556, 2008.
S. Ray, S. Jha, “Production of withaferin A in shoot cultures of Withania somnifera” Planta Medica. Vol.67, Issue. 2, pp. 432-436, 2001.
A.A.Kulkarni, S.R. Thengane, K.V. Krishnamurthy, “Direct shoot regeneration from node, internode, hypocotyl and embryo explants of Withania somnifera “ Plant Cell Tissue and Organ Culture. Vol. 62, Issue.3, pp. 203-209, 2000.
B.K.Ghimire, E.S. Seong, E.H.Kim et al., “Direct shoot organogenesis from petiole and leaf discs of Withania somnifera (L.) Dunal” African Journal of Biotechnology. Vol. 9, Issue.44, pp.7453-7461.
J.R. Rout, S.L.Sahoo, R. Das, “An attempt to conserve Withania somnifera (L.) Dunal – a highly essential medicinal plant through in vitro callus culture” Pakistan Journal of Botany. Vol. 43, Issue.4, pp. 1837-1842, 2011.
R.M.Taha and S.N. Wafa, “Plant regeneration and cellular behaviour studies in Celosia cristata grown in vivo and in vitro” The Scientific World Journal. Vol. 8, 2012.
N. Chakraborthy, D. Banerjee, M. Ghosh et al., “Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal” Physiology and Molecular Biology of Plants. Vol.19, Issue. 1, pp. 117-125, 2013.
V.S. Manickam, R.E. Mathavan, R. Antonisamy, “Regeneration of Indian ginseng plantlets from stem callus” Plant Cell Tissue and Organ Culture. Vol. 62, Issue. 3, pp. 181-185, 2000.
I. Sivanesan, “Direct regeneration from apical bud explants of Withania somnifera Dunal” Indian Journal of Biotechnology. Vol. 6, Issue 2, pp.125-127, 2000.
U. Supe, F. Dhote, M.G.Roymon, “In vitro plant regeneration of Withania somnifera” Plant Tissue Cult and Biotech. Vol. 16, Issue 2, pp.111-115, 2006.
K. Rao, B. Chodisetti, S. Gandi, S, L.N. Mangamoori , A. Giri, “Direct and indirect organogenesis of Alpinia galanga and the phytochemical analysis” Plant Cell.Tissue and Org. Culture. Vol.165, Issue. 5, pp. 1366-1378,2011.
K. Nasirujjaman, M. Salahuddin, S. Zaman, M.A. Reza, “Micropropagation of ginger (Zingiber officinale Var Rubrum using buds from microshoots" Journal of Biological Sciences. Vol.5, Issue. 4, pp. 490–492, 2005.
B. Gayathri, K.Rao, A. Giri, “Production of sapogenins (stigmasterol and hecogenin) from genetically transformed hairy root cultures of Chlorophytum borivilianum (Safed musli)” Plant Cell, Tissue and Organ Culture. Vol.131, pp. 369-376, 2017.
F. Sabir, R.S. Sangwan, L.Singh, L.N. Misra, N. Pathak, N.S. Sangwan, “Biotransformation of withanolides by cell suspension cultures of Withania somnifera (Dunal)” Plant Biotechnology Reports. Vol.5, Issue. 2, pp. 27-134, 2011.
J. Sen and A.K. Sharma, “Micropropagation of Withania somnifera from germinating seeds and shoot tips” Plant Cell Org. Tiss. Cult. Vol.26, pp. 71-73, 1999.
R. Udaykumar, C.W.Choi, K.T.Kim et al., “In vitro plant regeneration from epicotyl explant of Withania somnifera (L.) Dunal” Journal of Medicinal Plants Research. Vol. 7, Issue.1, pp. 43-52, 2013.
D.D.Shukla, N. Bhattarai, B. Pant, “In vitro mass propagation of Withania somnifera (L.) Dunal” Nepal Journal of Science and Technology. Vol.11, pp.101-106, 2011.
P.A.Wadegaonkar, K.A.Bhagwat, M.K. Rai, “Direct rhizogenesis and establishment of fast-growing normal root organ culture of Withania somnifera Dunal” Plant Cell Tissue and Organ Culture. Vol. 84, Issue. 2, pp. 223-225, 2006.
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