Production Kinetics of Xylanase and Carboxy-methyl Cellulase of Schizophyllum commune in Submerged Fermentation Technique

Authors

  • Kausik Majumder Department of Botany, Bidhannagar College, Government of West Bengal, Salt Lake City, Kolkata- 700064, India
  • Sumira Mukhia Post-Graduate Department of Botany, Darjeeling Government College, Darjeeling- 734101, India
  • Urmila Koirala Post-Graduate Department of Botany, Darjeeling Government College, Darjeeling- 734101, India

Keywords:

Tamarind kernel powder, Schizophyllum commune, xylanase, CMCase

Abstract

Tamarind kernel powder (TKP), a soluble agro-residue was used to evaluate the production of xylanase and CMCase in submerged culture of Schizophyllum commune, a white rot basidiomycetes fungi. Soluble TKP containing xyloglucan as the major polysaccharide induced significantly xylanase production but CMCase production was not significantly improved. The culture filtrate consisting of xylanase and CMCase at a ratio of 10:1 (approx) and 1.5:1(approx) in TKP and cellulose medium respectively. It may therefore, be indicative that inducer molecules released from TKP or cellulose were not identical and Tamarind kernel powder, although structurally similar to cellulose, therefore, was found to be the perfect inducer for xylanase production in submerged culture of Schizophyllum commune. Use of mixed substrate in a single set of fermentation reveals that addition of 0.5 % TKP in cellulose medium improved CMCase titer, whereas addition of 0.5 % cellulose in TKP medium did not increased the production level of xylanase. Therefore, the present study reports the successful economic utilization of TKP, an abundantly available for the production of xylanase, an enzyme of industrial importance.

 

References

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Published

2019-04-30

How to Cite

[1]
K. Majumder, S. Mukhia, and U. Koirala, “Production Kinetics of Xylanase and Carboxy-methyl Cellulase of Schizophyllum commune in Submerged Fermentation Technique”, Int. J. Sci. Res. Biol. Sci., vol. 6, no. 2, pp. 81–85, Apr. 2019.

Issue

Vol. 6 No. 2 (2019): April Edition

Section

Research Article

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