Production and optimization of Polyhydroxybutyrate and its application in bioplastic production
Keywords:
PHB, Nitrogen deficient medium, Modified LB medium, FTIR, TLCAbstract
Polyhydroxybutyrate (PHB) is a natural biopolymer by wide range of microbes which is a storage granule used by microbes as an energy as well as carbon source. PHB exhibit stiffness, brittle to rubber like, biodegradable, environment friendly properties. In present work isolates was produced Polyhydroxybutyrate by using Nitrogen deficient medium and modified LB medium with glucose as carbon source. Maximum production was observed in Modified LB medium as compared to nitrogen deficient medium. Extraction of bacterial PHB was done by sodium hypochlorite method; selected bacteria produced 2.10g/L PHB .Confirmation of PHB molecule was by FTIR & TLC. Extracted bacterial PHB was capable to produce biodegradable plastic which shows water tolerance activity. PHB production was found to be maximum at optimum pH 7.5, 37°C, 3% inoculums percentage and glucose concentration 20g/L.
References
Anish Kumari Bhuwal,1Gulab Singh, Neeraj Kumar Aggarwal, Varsha Goyal& Anita Yadav, “ Isolation and Screening of Polyhyroxyalkanoates Producing Bacteria From Pulp, Paper, and Cardboard Industry Waste”. Hindawi Publishing Corporation International Journal of Biomaterials, Vol. 2013, 752821, pp. 1-10, 2013.
Abdullah A. Alarfaj, Mohammed Arshad, Essam N. Sholkamy, Murugam A. Munusamy, “Extraction and Characterization of Polyhydroxybutyrate (PHB) from Bacillus thuringiensis KSADL127 Isolated from Mangrove Environments of Saudi Arabia.” Brazilian Archives of Biology and Technology, Vol. 58, Issue., 5, 2015.
Jehan Mohammed El- Mohamedy Hawas, Tarek El-Said El- Banna, ElsayedBelalAbdelmontelebBelal, et.al., “Production of Bioplastic from some Selected Bacterial Strains.” International Journal of Current Microbiology and Applied Sciences, Vol. 5, Issue., 1, pp. 10-22, 2016.
Akram Hoceinabadi1, Iraj Rasooli, MojtabaTaran, “Isolation and Identification of Poly β - Hydroxybutyrate Over-Producing Bacteria and Optimization of Producing Medium”. Jundishapur J Microbiol, Vol. 8, Issue., 7: e16965, 2015.
Neelam K. Naranje, Bharat J. Wadher, Hemant J. Purohit, Manohar G. Muddeshwar, “Bacillus megaterium as potential producer for Polyhydroxybutyrate.” IOSR Journal of Environmental Science, Toxicology and Food Technology, Vol. 1, Issue., 6, pp. 07-10, 2015.
S. Kumaravel, R. Hema & R. Lakshmi, “Production of Polyhydroxybutyrate (bioplastic) and its Biodegradation by Pseudomonas lemoignei and Aspergillusniger.” E Journal of Chemistry, Vol. 7, Issue.,S1, pp. S536-S542, 2010.
Gupta Shraddha, Rajput Yogita, Shit Simanta, ShuklaAparna, ShuklaKamlesh, “Screening and Production of bioplastic (PHAs) from Sugarcane Rhizospheric Bacteria.” International Multidisciplinary Research Journal, Vol. 1, Issue., 9, pp. 30-33, 2011.
B. Senthil Kumar and G Prabhakarn, “Production of PHB (bioplastic) using bio-effluents substrate by Alcaligens eutrophus.” Indian Journal of Biotechnology, Vol. 5, pp. 76-79, 2006.
Anteneh Getachew and Fantahun Woldesenbet “Production of biodegradable plastic by Polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material.” Getachew and Woldesendet BMC Res Notes, Vol. 9, pp. 501-509, 2016.
Xiao-Ran Jiang, Huan Wang, Guo-Qiang Chen, “Engineering the bacterial shapes for enhanced inclusion bodies accumulation” Metabolic Engineering, Vol. 29, pp. 227-237, 2015.
Gulab Singh, Anish Kumari, Aparna Mittal, Anita Yadav & Neeraj k. Aggarwal, “Poly β-Hydroxybutyrate Production by Bacillus subtilis NG 220 Using Sugar Industry Waste Water.” Hindawi Publishing Corporation International Journal of Biomaterials, Vol. 2013, 952641, pp. 1-10, 2013.
Robert Findlay & David C. White, “Polymeric Beta-Hydroxyalkanoates from Environmental Samples and Bacillus megaterium, Applied and Environmental Microbiology, Vol. 45, pp. 71-78, 1983.
S. Sujatha and D. Ravi, “A study on poly hydroxy butyrate (PHB) production and its application from Pseudomonas species (MTCC).” Biosciences, Biotechnology Research Asia, Vol.7, Issue., 2, pp. 825 -832, 2010.
Prayashree Bahalia, “Estimation of Kinetic Parameters for Production of PHB”.
Wala’a Shawkat Ali, Neihaya Heikmat Zaki and Somaya Yassen Najim Obiad “Production of bioplastic by bacteria isolated from local soil and organic wastes.” Current Research in Microbiology and Biotechnology Vol. 5, Issue., 2, pp. 1012-1017, 2017.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors contributing to this journal agree to publish their articles under the Creative Commons Attribution 4.0 International License, allowing third parties to share their work (copy, distribute, transmit) and to adapt it, under the condition that the authors are given credit and that in the event of reuse or distribution, the terms of this license are made clear.
Download Article Template
