Rhizobacterial isolates of Capsicum chinense inhibited fungal pathogen Rhizoctonia solani
Keywords:
Rhizobacteria, Umorok, Antagonism, Molecular characterization, Rhizoctonia solaniAbstract
Umorok (Capsicum chinense) is a commercially important chilli plant of Northeastern India. To improve the overall growth and health of the plant we explore the rhizosphere bacteria associated with the plant through serial dilution and pure culture method from three different growth stages juvenile, flowering and fruiting stage. The isolated rhizobacteria were then screened for their biocontrol enzyme activities like cellulose, protease and chitinase, and the potential isolates were molecular characterized using 16S rRNA gene sequencing. The identified microorganism were then studied for its antagonism assay against the fungal pathogen Rhizoctonia solani using dual culture method. The isolates which showed the highest antagonism were selected and treated with the pathogen infected Umorok plant and measured the growth indicating traits in the greenhouse experiment. During the molecular characterization of the rhizobacteria, 127 novel strains were identified. Five bacterial phyla were observed in the three growth stages, and gammaproteobacteria were predominantly present among them. Seven potential rhizobacteria were selected from the diverse list of rhizobacteria showing the biocontrol and antagonism assays. Umorok was infected with plant pathogen R. solani and treated with the selected rhizobacteria to measure the plant growth. The findings confirmed that Lysobacter enzymogenesis competitively inhibited the R. solani and significantly improved the shoot and root system.
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