Antibacterial Sctivity of Fresh Juices of Lemon, Onion, Bottle Gourd and Tomato Against Multiple Drug Resistant Bacteria such as E.Coli, Staphylococcus, Bacillus, Klebsiella and Salmonella
Keywords:
Agar Well Diffusion, Culture, Antimicrobial Activity, ResistantAbstract
Antibiotic resistant bacteria are one of the major problems challenging the health care system. Over three-quarter of the world’s population is using herbal medicines with an increasing trend globally. Plant medicines may be beneficial but are not completely harmless. The aim of this study is to evaluate the antimicrobial activity of different vegetables against different microbial cultures. In this experiment undiluted crude extract and aqueous suspensions (1:10) of Allium cepa (onion), Citrus lemon (lemon), Lagenaria siceraria (bottle gourd), lycopersicon esculentum (Tomato) extracts were selected for anti-microbial activity using agar-well diffusion method. It is tested against Staphylococcus, E.coli, klebsiella, bacillus and Salmonella. All the bacteria are susceptible to all above extracts with the diameter of zones of inhibition ranging from 11mm –29mm. The juice of lemon has antimicrobial activities more than other types of extracts whereas juice of bottle gourd does not show any antimicrobial activity against drug-resistant bacteria. Their activity against test bacteria found in following order- Lemon, onion, tomato and bottle gourd. All pathogens are susceptible. The antimicrobial activity has been checked in terms of MIC by using different dilutions. All the extracts (Lemon, Tomato, Onion and Gourd) possess anti-oxidant, anti-bacterial and anti-inflammatory activity. It could be therefore concluded that the consumption of these vegetables would exert both nutritional and health benefits by virtue of their antioxidant, antibacterial and anti-inflammatory activity. In this review article I have tried to provide a survey of the literature on the scientific researches of antimicrobial properties of these wonderful herbal drugs.
References
Ahmad, M.M., Z. Salim-ur-Rehman, F.M. Iqbal-Anjumand J.I. Sultan, 2006. Genetic variability to essentialoil composition in four citrus fruit species. Pak. J.Bot., 38(2): 319-324.
Al-Shuneigat, J., S.D. Cox and J.L. Markham, 2005.Effects of a topical essential oil containing formulation on biofilm-forming coagulase-negative staphylococci. Lett. Appl. Microbiol., 41: 52-55.
Burt, S.A., 2004. Essential oils: Their antibacterial properties and potential applications in foods: Areview. Inter. J. Food Microbiol., 94: 223-253.
Dorman, H.J. and S.G. Deans, 2000. Antimicrobial agents from plant: Antibacterial activity of plant volatile oils. J. Appl. Microbiol., 88: 308-316.
Duthie, G. and A. Crozier, 2000. Plant-derived phenolic antioxidants. Curr. Opin. Lipidol., 11: 43-47.
Gislene, G.F., N.J. Locatelli, C.F. Paulo and L.S. Giuliana, 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Braz. J. Microbiol., 31: 247-256.
Kawaii, S., T. Yasuhiko, K. Eriko, O. Kazunori,Y. Masamichi, K. Meisaku, ChihiroIto and F. Hiroshi, 2000. Quantitative study of flavonoids in leaves of Citrus plants J. Agric. Food Chem., 48:3865-3871.
Gerber C, History of herbal medicine, Modified September 30, 2008. [cited2010Oct13]. Available at :http://herbs.lovetoknow.com/History_HerbalMedicine.
Grunnet J, Advantages and disadvantages of herbal medicine, Modified May13, 2010,.Available at :http://herbs.lovetoknow.com/Advantages_and_Disadvantages_of_Herbal_Medicine
Akesbi, N. (2006) évolution et perspectives de l’agriculture marocaine. Rapport 50 Ans De Développement Humain Et Perspectives, 2025, 85-198.
Hernández Suárez, M., Rodríguez Rodríguez, E.M. and Díaz Romero, C. (2008) Chemical composition of tomato (Lycopersicon esculentum) from Tenerife, the Canary Islands. Food Chemistry, 106, 1046-1056. doi:10.1016/j.foodchem.2007.07.025
Olaniyi, J.O., Akanbi, W.B., Adejumo, T.A. and Akande, O.G. (2010) Growth fruit yield and nutritional quality of tomato varieties. African Journal of Food Science, 4, 398-402.
Bashan, Y. and De-Bashan, L.E. (2002) Protection of tomato seedlings against infection by Pseudomonas syringae pv. tomato by using the plant growth-promoting bacterium Azospirillum brasilense. Applied and Environmental Microbiology, 68, 2637-2643. doi:10.1128/AEM.68.6.2637-2643.2002
Alipourfard, I., Yeasmin, N.N. (2010).Antibiogram of extended spectrum beta-lactamase producing Escherichia coli and Klebsiella pneumoniae isolated from hospital samples. Bangladesh J Med Microbiol, 04(01):32-36.
Bergey, D.H., John, G.H. (1994). Bergey’s manual of deter-minative biology, William and Wilkins, 9 ed., Chapter 4, Pp. 181-186.
Jahan, T., Begum, Z.A., and Sultana, S. (2007). Effect of neem oil on some pathogenic bacteria. Bangladesh Journal of Pharmacology. 2 (2): 71-72
Batista, O., Duarte,A., Nascimento, J. and Simones, M.F. (1994). Structure and antimicrobial activity of diterpenes from the roots of Plectranthus hereroensis. J. of Nat. Product.57: 858-861.
Chopra. R. N., Nayar. S. L. and Chopra, I. C. (1986). Glossary of Indian Medicinal Plants (Including the Supplement). Council of Scientific and Industrial Research, New Delhi.
Cichewicz, R.H. and P.A. Thorpe (1996). the antimicrobialproperties of the chile pepers (Copsicum species) and their uses in Mayan Medicine. J. Ethanopharmacol., 52: 61-70.
Decker-Walters, D.S., Wilkins-Ellert, M., Chung, S.-M. and Staub, J.E. (2004). Discovery and genetic assessment of wild bottle gourd [Lagenaria siceraria Standley, Cucurbitaceae] from Zimbabwe . Economic Botany, 58: 501-508.
Deshponde, J.R., A.A. Choudhari, M.R.Mishra, V.S. Meghre,S.G.Wadodkar and A. K. Dorle (2008). Beneficial effects of Legenaria siceraria (Mol.) Standley fruit epicarp in animal models. Ind. J. of Exptl. Biol. 46: 234-242
Warrier P.K., Nambiar, V. P. K and Ramnkutty, C. (1995). Legenaria siceraria (Mol.) Standley, in indian medicinal plants. Vol. 3, 292. Orient Longman Limited, Madras, India
Azu,N.c, and Onyeagba,R.A. (2007). Antimicrobial properties of extract of Allium cepa (onions) and Zingiber officinale (ginger) on Escherichia coli, Sallmonella typhi, Bacillus subtillus. The interned j. of tropical medicine.3.2.
Cavallito,C.J. & Bailey.J.H. (1944). Allium the antibiotic principle of allium sativum.isolation,physical properties & antibacterial action.J.Am.Soc.66:190 –195.
Chen,H.C., Chang,T.J. (1985). Antibacterial properties of some spice plant before and after heat treatment. Pubmed, 18(3):190 –195
Hughes,B.G. & Lawson,L. (1991). Antimicrobial effects of allium sativum & allium cepa, garlic compound & commercial garlic supplement product. phytother Res; 5:154 –158
De Castillo, M.C.; De Allori, C.G.; De Gutierrez, R.C.; De Saab, O.A.; De Fernandez, N.P.; De Ruiz, C.S.; De Ruiz Holgado, A.P. and De Nader, OM. (2000). Bactericidal activity of Lemon juice and Lemon derivatives against Vibrio cholerae. Boil. Pharm. Bull.,23:1235-1238.
Anesini,E. and Perez, C. (1993). Screening of plants used in Argentine folk medicine for antimicrobial activity. J. Ethnopharmacol. 39: 119-128.
Downloads
Published
How to Cite
Issue
Section
License

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors contributing to this journal agree to publish their articles under the Creative Commons Attribution 4.0 International License, allowing third parties to share their work (copy, distribute, transmit) and to adapt it, under the condition that the authors are given credit and that in the event of reuse or distribution, the terms of this license are made clear.